LOCUS       sequence-134136-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloned region contains Acetobacter aceti 1023 purE(H59D) gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK346
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(599..615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(599..615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(612..634)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    623..639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(647..653,654..671,672..677))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    692..713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(830..847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttatcttcgg taatgggaga gttggggaca gaggccgtct gcaatgcgcg ccatgtttca
       61 aggcgtgcgg caagggccgg gttatacagt gcaagaatgg aggcggccag caaggcggca
      121 tttttggcgc cggatgcccc aatagccaaa gtgcccacag gcacgccgcc gggcatctgc
      181 acaatggaaa gcaggctgtc catgccttta agagcgcgag attctactgg aacacccagc
      241 acgggcaggc gtgtccatgc cgcacacata cccggcagat gtgcagctcc accggcccca
      301 gcaataatca cattcaggcc gcgttcggct gccgtacgcg cataatccgc caaccggtct
      361 ggcgtacgat ctgcagaaac aatgagggtt tcatgcggaa tttccagctc tgtcagcaat
      421 gcgtctgcat ggcgcatggt ttcccagtct gactggctgc ccatgataat gcccacaacg
      481 ggggcagagg ctgctttatc ttctaaagca ctggaagcag agggcagggg ggcggtttcg
      541 ctcacggtgg gtatctatcc ggttttttaa agtctgtaac ggaggaattc gtaatcatgt
      601 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      661 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      721 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      781 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      841 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      901 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      961 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1021 ctgac
//
