LOCUS       sequence-134149-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combines a gCaMP-based calcium indicator and an Arch-based voltage 
            indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Dempsey GT, Chaudhary KW, Atwater N, Nguyen C, Brown BS, McNeish JD,
            Cohen AE, Kralj JM
  TITLE     Cardiotoxicity screening with simultaneous optogenetic pacing, 
            voltage imaging and calcium imaging.
  JOURNAL   J Pharmacol Toxicol Methods. 2016 May 13. pii: 
            S1056-8719(16)30044-2. doi: 10.1016/j.vascn.2016.05.003.
  PUBMED    27184445
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Pharmacol
            Toxicol Methods. 2016 May 13. pii: S1056-8719(16)30044-2. doi: 
            10.1016/j.vascn.2016.05.003."
COMMENT     Sequence Label: pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      408..417
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             417..458
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gttggaggtg ggggaggtag gaagagcgat gatcacttgt ggactaagtt tgttcgcatc
       61 cccttctcca accccctcag tacatcaccc tgggggaaca gggtccactt gctcctgggc
      121 ccacacagtc ctgcagtatt gtgtatataa ggccagggca aagaggagca ggttttaaag
      181 tgaaaggcag gcaggtgttg gggaggcagt taccggggca acgggaacag ggcgtttcgg
      241 aggtggttgc catggggacc tggatgctga cgaaggctcg cgaggctgtg agcagccaca
      301 gtgccctgct cagaagcccc aagctcgtca gtcaagccgg ttctccgttt gcactcagga
      361 gcacgggcag gcgagtggcc cctaattctg ggggcagcgg gggatccgcc accatgggca
      421 agcccatccc caaccccctg ctgggcctgg acagcaccga ccccatcgct ctgcaggctg
      481 gttacgacct actgggtgac ggcagacctg aaaccctgtg gctgggcatc ggcactctgc
      541 tgatgctgat tggaaccttc tactttctgg tccgcggatg gggagtcacc gataaggatg
      601 cccgggaata ttacgctgtg actatcctgg tgtccggaat cgcatccgcc gcatatctgt
      661 ctatgttctt tggtatcggg cttactgagg tgtccgtcgg gggcgaaatg ttggatatct
      721 attatgccag gtacgcccaa tggctgttta ccaccccact tctgctgctg cacctggccc
      781 ttctcgctaa ggtggatcgg gtgaccatcg gcaccctggt gggtgtggac gccctgatga
      841 tcgtcactgg cctcatcgga
//
