LOCUS       sequence-134151-       11527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Combines a gCaMP-based calcium indicator and an Arch-based voltage 
            indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Dempsey GT, Chaudhary KW, Atwater N, Nguyen C, Brown BS, McNeish JD,
            Cohen AE, Kralj JM
  TITLE     Cardiotoxicity screening with simultaneous optogenetic pacing, 
            voltage imaging and calcium imaging.
  JOURNAL   J Pharmacol Toxicol Methods. 2016 May 13. pii: 
            S1056-8719(16)30044-2. doi: 10.1016/j.vascn.2016.05.003.
  PUBMED    27184445
REFERENCE   2  (bases 1 to 11527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Pharmacol
            Toxicol Methods. 2016 May 13. pii: S1056-8719(16)30044-2. doi: 
            10.1016/j.vascn.2016.05.003."
COMMENT     Sequence Label: pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..11527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3890
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3900..3909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3909..3950
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3951..4721
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="DPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVTD
                     KDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLLL
                     HLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLAT
                     SLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLDV
                     TAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
     CDS             join(4815..4823,4824..4841,4842..4844,4845..4877,4878..4880,
                     4881..4904,4905..4931,4932..4988,4989..4994,4995..5264,
                     5265..5282,5283..5717,5718..5723,5724..6164)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      4911..4920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5201..5222
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5328..5349)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5589..5608)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    6219..6807
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6272..6292)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6690..6701)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6810..6826)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6811..6827)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7332..7512
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7538..7555)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7544..7768
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7814..8242
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7901..7920)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8111..8132
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8251..8271)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8256..8585
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8436..8571
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8498..8517
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8633..8680
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8699..9073
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9203..9324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9240..9259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9294..9313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9373..9389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9373..9389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9386..9408)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9397..9413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9421..9427,9428..9445,9446..9451))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9466..9487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9604..9621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9775..10363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9855..9874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10534..11325,11326..11394))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11157..11176
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11395..11499)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctaattc tgggggcagc gggggatccg
     3901 ccaccatggg caagcccatc cccaaccccc tgctgggcct ggacagcacc gaccccatcg
     3961 ctctgcaggc tggttacgac ctactgggtg acggcagacc tgaaaccctg tggctgggca
     4021 tcggcactct gctgatgctg attggaacct tctactttct ggtccgcgga tggggagtca
     4081 ccgataagga tgcccgggaa tattacgctg tgactatcct ggtgtccgga atcgcatccg
     4141 ccgcatatct gtctatgttc tttggtatcg ggcttactga ggtgtccgtc gggggcgaaa
     4201 tgttggatat ctattatgcc aggtacgccc aatggctgtt taccacccca cttctgctgc
     4261 tgcacctggc ccttctcgct aaggtggatc gggtgaccat cggcaccctg gtgggtgtgg
     4321 acgccctgat gatcgtcact ggcctcatcg gagccttgag ccacacggcc atagccagat
     4381 acagttggtg gttgttctct acaatttgca tgatagtggt gctctatgtt ctggctacat
     4441 ccctgcgatc tgctgcaaag gagcggggcc ccgaggtggc atctaccttt aacaccctga
     4501 cagctctggt cttggtgctg tggaccgctt accctatcct gtggatcata ggcactgagg
     4561 gcgctggcgt ggtgggcctg ggcatcgaaa ctctgctgtt tatggtgttg gacgtgactg
     4621 ccaaggtcgg ctttggcttt atcctgttga gatcccgggc tattctgggc gacaccgagg
     4681 caccagaacc cagtgccggt gccgatgtca gtgccgccga ccgaccggta gtagcagtga
     4741 gcaaggcggc cgccaagagc aggatcacca gcgagggcga gtacatcccc ctggaccaga
     4801 tcgacatcaa cgtgatgggt tctcatcatc atcatcatca tggtatggct agcatgactg
     4861 gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggatctc gccaccatgg
     4921 tcgactcatc acgtcgtaag tggaataaga caggtcacgc agtcagagct ataggtcggc
     4981 tgagctcact cgagaacgtc tatatcaagg ccgacaagca gaagaacggc atcaaggcga
     5041 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgcctac cactaccagc
     5101 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcgtgc
     5161 agtccaaact ttcgaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5221 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt accggaggga
     5281 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5341 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc gatgccacct
     5401 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5461 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5521 agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag cgcaccatct
     5581 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5641 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5701 acaagctgga gtacaacctg ccggaccaac tgactgaaga gcagatcgca gaatttaaag
     5761 aggaattctc cctatttgac aaggacgggg atgggacaat aacaaccaag gagctgggga
     5821 cggtgatgcg gtctctgggg cagaacccca cagaagcaga gctgcaggac atgatcaatg
     5881 aagtagatgc cgacggtgac ggcacaatcg acttccctga gttcctgaca atgatggcaa
     5941 gaaaaatgaa atacagggac acggaagaag aaattagaga agcgttcggt gtgtttgata
     6001 aggatggcaa tggctacatc agtgcagcag agcttcgcca cgtgatgaca aaccttggag
     6061 agaagttaac agatgaagag gttgatgaaa tgatcaggga agcagacatc gatggggatg
     6121 gtcaggtaaa ctacgaagag tttgtacaaa tgatgacagc gaagtacaag ttctgctacg
     6181 agaacgaggt gtaagtcgac gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     6241 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6301 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6361 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6421 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6481 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6541 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6601 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     6661 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6721 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6781 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     6841 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     6901 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     6961 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7021 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7081 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     7141 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     7201 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7261 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     7321 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7381 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7441 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7501 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     7561 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7621 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7681 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7741 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     7801 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7861 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7921 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7981 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8041 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8101 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8161 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8221 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8281 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8341 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8401 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8461 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8521 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8581 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     8641 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     8701 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     8761 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     8821 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     8881 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     8941 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     9001 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     9061 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     9121 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9181 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     9241 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9301 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     9361 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9421 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9481 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9541 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9601 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9661 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9721 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9781 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9841 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9901 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9961 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10021 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10081 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10141 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10201 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10261 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10321 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10381 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10441 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10501 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10561 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10621 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10681 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10741 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10801 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10861 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10921 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10981 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    11041 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    11101 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11161 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11221 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11281 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11341 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11401 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11461 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11521 acctgac
//
