LOCUS       sequence-134152-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA to the vesicular GABA transporter VGAT. Cre 
            dependent expression by flex switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Yu X, Ye Z, Houston CM, Zecharia AY, Ma Y, Zhang Z, Uygun DS, Parker
            S, Vyssotski AL, Yustos R, Franks NP, Brickley SG, Wisden W
  TITLE     Wakefulness Is Governed by GABA and Histamine Cotransmission.
  JOURNAL   Neuron. 2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003.
  PUBMED    26094607
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003."
COMMENT     Sequence Label: pAAV-hsyn-flex-dsRed-shvgat
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(124..157)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(311..329)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(361..381)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     579..598
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     773..796
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta tagcatacat
       61 tatacgaagt tattctttgc accattctaa agaataacag tgataatttc tgggttaagg
      121 caaataactt cgtataggat actttatacg aagttatgct agcgctaccg ggcctgcagg
      181 atggactcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
      241 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      301 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      361 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
      421 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      481 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggctc cttcatctac
      541 aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca gaagaagact
      601 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
      661 atccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc
      721 tacatggcca agaagcccgt gcagctgccc ggctactact acgtggactc caagctggac
      781 atcacctccc acaacgagga ctacaccatc gtggagcagt a
//
