LOCUS       sequence-134160-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-Mnase expressed under the REB1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S
  TITLE     ChEC-seq kinetics discriminates transcription factor binding sites 
            by DNA sequence and shape in vivo.
  JOURNAL   Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.
  PUBMED    26490019
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-22"; volume: "6:8733. doi"; pages: " 
            10.1038/ncomms9733"
COMMENT     Sequence Label: pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     63..79
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(105..125)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(129..575)
                     /codon_start=1
                     /product="micrococcal nuclease from Staphylococcus aureus"
                     /label=MNase
                     /translation="ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETK
                     HPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALV
                     RQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ"
     CDS             complement(582..647)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
       61 gctctagaac tagtggatcc cccgggctgc aggaattcct actaaacttt tctttttttt
      121 tttggtggct ggccgctatc ggcgttatcc tcactccaga tgttcagttt ctccttcttt
      181 gcctgagcct cgctcttgcg caggtgctgc tcgtgggtgt tgttgggctt gtacacgtac
      241 gccaccttgg ccagtccctg ccgcaccagg gcctcgttca ccattttgcc gtcggcgtag
      301 atgtaggcca gaccgcgtcc atacttatcg gtgcgttggc ctttatcgaa ctccacctcg
      361 attttcttgg cgttctccac catctttttt gtgaaggcgc tggcctcggg tccgtacttt
      421 tccacgccct ttttcgggtg cttggtctcg ggggtatcca ccagcagcag gcggaaggtc
      481 atgggctggc ccttatacat cagcttcacg gtatcgccat ctatggcctt gatcagggtg
      541 gcgggctcct tgtgcagctt cttggtactg gtggcggccg ccttatcatc atcatccttg
      601 taatcgatat cgtgatcctt gtaatcgcca tcgtgatcct tgtaatccat attgattgag
      661 ctataaatat tctaattcag cancaacacg tgagttcaaa ctaataacga tagtgtgtag
      721 ataaccttgc ttgtgatga
//
