LOCUS       sequence-134183-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloned region contains Acetobacter aceti 1023 purE gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK324
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             575..583
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(599..616)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(599..615)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(608..630)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(915..934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttaaaaaac cggatagata cccaccgtga gcgaaaccgc ccccctgccc tctgcttcca
       61 gtgctttaga agataaagca gcctctgccc ccgttgtggg cattatcatg ggcagccagt
      121 cagactggga aaccatgcgc catgcagacg cattgctgac agagctggaa attccgcatg
      181 aaaccctcat tgtttcagca catcgtacgc cagaccggtt ggcggattat gcgcgtacgg
      241 cagccgaacg cggcctgaat gtgattattg ctggggccgg tggagctgca catctgccgg
      301 gtatgtgtgc ggcatggaca cgcctgcccg tgctgggtgt tccagtagaa tctcgcgctc
      361 ttaaaggcat ggacagcctg ctttccattg tgcagatgcc cggcggcgtg cctgtgggca
      421 ctttggctat tggggcatcc ggcgccaaaa atgccgcctt gctggccgcc tccattcttg
      481 cactgtataa cccggccctt gccgcacgcc ttgaaacatg gcgcgcattg cagacggcct
      541 ctgtccccaa ctctcccatt accgaagata aatgacctcc ttacattcta agcttggcac
      601 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      661 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      721 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
      781 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
      841 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      901 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccgg
//
