LOCUS       sequence-134205-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  antisense probe for detection of human SNORD60 (C/D box snoRNA U60).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pcDNA3.1_SNORD60 probe
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             201..242
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             252..269
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(292..309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    298..522
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      568..925
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(655..674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agcttggtac cgagctcgga tccactagtc cagtgtggtg gaattgccct taagcctcag
       61 tcttgctaaa taatcagact gcacgtgcag ttttcatacg aggtgtcaga agtcaaagca
      121 attcatcaca gactaagggc aattctgcag atatccagca cagtggcggc cgctcgagtc
      181 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
      241 cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
      301 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      361 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
      421 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
      481 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
      541 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
      601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      721 gggggctccc tttanggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      781 attanggtga tggttcacgt antgggccat cgccctgata gacggttttt cgccctttga
      841 cgttggagtc cacgttcttt aatagtggac tcttgttcnn aactggaaca nnactcnacc
      901 ctatctcggt ctattctttg atttatnngg nntttgccga tttcggncnn antggttaaa
      961 aaatgagctg anttaacaaa aatttaaccg cgaattantt c
//
