LOCUS       sequence-134210-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Phe79>Trp mutant (TdPurE2-F79W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Kappock
  TITLE     Single Trp variants of Acetobacter aceti PurE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK648
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             608..630
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(661..680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(662..680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcgag ctccgtcgac aagaaactga aacgctgaat
      121 tcagcgtttc agtttcgaat cgtcttcgat aattttctgc gcattggatt ccatatagga
      181 tttgaccgaa tctgcgattt ctttatcgta cagtgaaaaa atacgagccg ccaggagtgc
      241 cgcatttttc ggttcaagaa ccagtgccgg actaatcccg ctcggcatcc gtaacgaaga
      301 ataaatgtcg gcgccggcga agctatcgct tggcggtggg caggcaatgg ttgcaccttt
      361 gacccagcca tcaacaaagc ctgataaagc gttactacgc ccagcgatcg tgatgtacag
      421 tttaggacgg tccaaagctt catattcttt cagcatagac actacatgct ccgctgtttt
      481 atgcgcggag ccgatgcgaa tggcatactc aataccaaaa gttttcaatt cagacgcaat
      541 tttctcggcg tgacccatat ctgaactgct gcccataagg ataatcacca gcggacgcat
      601 atgtatatct ccttcttaaa gttaaacaaa attatttcta gagggaaacc gttgtggtct
      661 ccctatagtg agtcgtatta atttcgcggg atcgagatct cgggcagcgt tgggtcctgg
      721 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
      781 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
      841 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
      901 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
      961 tgctggctac cctgtggaac acctacatct gtattaacga
//
