LOCUS       sequence-134240-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Ser38>Val/Phe79>Trp mutant (TdPurE2-S38V,F79W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK656
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             607..629
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(660..679)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(661..679)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcgagc tccgtcgaca agaaactgaa acgctgaatt
      121 cagcgtttca gtttcgaatc gtcttcgata attttctgcg cattggattc catataggat
      181 ttgaccgaat ctgcgatttc tttatcgtac agtgaaaaaa tacgagccgc caggagtgcc
      241 gcatttttcg gttcaagaac cagtgccgga ctaatcccgc tcggcatccg taacgaagaa
      301 taaatgtcgg cgccggcgaa gctatcgctt ggcggtgggc aggcaatggt tgcacctttg
      361 acccagccat caacaaagcc tgataaagcg ttactacgcc cagcgatcgt gatgtacagt
      421 ttaggacggt ccaaagcttc atattctttc agcatagaca ctacatgctc cgctgtttta
      481 tgcgctacgc cgatgcgaat ggcatactca ataccaaaag ttttcaattc agacgcaatt
      541 ttctcggcgt gacccatatc tgaactgctg cccataagga taatcaccag cggacgcata
      601 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc
      661 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc
      721 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
      781 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
      841 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
      901 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
      961 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
     1021 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
     1081 gtaaccgggc atgttcatca tcagtaa
//
