LOCUS       sequence-134319-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Shaw RJ, Kosmatka M, Bardeesy N, Hurley RL, Witters LA, DePinho RA, 
            Cantley LC
  TITLE     The tumor suppressor LKB1 kinase directly activates AMP-activated 
            kinase and regulates apoptosis in response to energy stress.
  JOURNAL   Proc Natl Acad Sci U S A 2004 Mar 9;101(10):3329-35. Epub 2004 Feb 
            25.
  PUBMED    14985505
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "2004-03-9"; volume: "101(10)"; pages: 
            "3329-35. Epub 2004 Feb 25"
COMMENT     Sequence Label: pcDNA3-FLAG-LKB1
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    119..343
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     332..349
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        369..387
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     369..386
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
       61 ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc ctcagaagcc
      121 atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg
      181 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt
      241 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg
      301 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcga
      361 gctctagcat ttaggtgaca ctatagaata gggccctcta gatgcatgct cgacgcctca
      421 ctgctgcttg caggccgaca gccggcggat cttgctgctg gcggagcagg ccttgcgggc
      481 agggttgggg gcccggccct ccgccctgga tttggtgctc agctgcgccg cctctgtgcc
      541 gttcatacac acggccttgg ggaggccccg gcgctgtcca ttgtgactgg cctcctcttc
      601 tgggacctgt ccgggcaccg tgaagtcctg agtgtagatg atgtcatcct cgatgtcgaa
      661 gaggtcctcg tcctcgtccg cgccgtgcag gtcctccaag tacggcacca cagtcatgct
      721 gcgccaccgg tccttggtgt ctgggctcgg tgggatgggc actggtgctt cagccggagg
      781 atgtttcttc cggaaccagc tgtgctgccg gatctgccgg atggagaacc tcttggccgg
      841 ttcgtactca agcatccctt tcagcaggtc agagagcggg gggccacagt cgcccgggat
      901 ggcgtagctc cccttcccga tgttctcaaa caacttgtag atgttgtccc cttcgaaggg
      961 gtacagaccc gtggtgatgt tgtagagggt gaccccagcc gaccagatgt ccaccttgaa
     1021 gccggagaag gtgtccaggc cgttggcaat ctcgggcggc tggaaagccg gggagccctg
     1081 gctggtccgg caggtgtcgt ccgccgcgaa
//
