LOCUS       sequence-134401-         449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Production of Acetobacter aceti 1023 thioredoxin (AaTrx).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Starks CM, Francois JA, MacArthur KM, Heard BZ, Kappock TJ
  TITLE     Atomic-resolution crystal structure of thioredoxin from the 
            acidophilic bacterium Acetobacter aceti.
  JOURNAL   Protein Sci. 2007 Jan;16(1):92-8.
  PUBMED    17192591
REFERENCE   2  (bases 1 to 449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2007-01"; volume: "16(1)"; pages: "92-8"
COMMENT     Sequence Label: pJK295
FEATURES             Location/Qualifiers
     source          1..449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             302..319
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(372..390)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      386..433
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gtgctggtag atttctgggc agaatggtgc ggcccatgca aaatgattgg cccggcattg
       61 ggagaaattg gcaaggaatt tgccggcaag gtaacggttg ccaaggtaaa tattgatgac
      121 aacccggaaa ctccaaatgc gtatcaggtg cgtagcattc caacccttat gctggtgcgt
      181 gatggcaagg ttatagataa aaaagtgggt gccctgccca aaagccagtt gaaggcatgg
      241 gtagaaagcg ctcagtaatt agaattcgag ctccgtcgac aagcttgcgg ccgcactcga
      301 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      361 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      421 gaggggtttt ttgctgaaag gaggaacta
//
