LOCUS       sequence-134495-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a 
            C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK587
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1003..1025
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1056..1075)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1057..1075)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagttctcc gctcaaacca
       61 gcaagatacc gttccgcatc tagcgcagcc atacagccag tcccagccgc agtgacggcc
      121 tggcggtatg ttttgtcctg cacatccccc gcggcaaaga ctccgggcac agatgtacgt
      181 gtggtgccgg gtgtggtttc aatataccct tcagcatcca gagtaagctg gttgcggaag
      241 atgcctgtgt taggggcgtg cccaatggca ataaatacgc catcggtcga gacagtctgt
      301 tcgctattat cctgcgtatt cttgagccgc acaccgcata cggtttctgg cgttccgtcg
      361 gccagaatat cttgcaccac gctgttccag atcaccgaaa ccttcgggtt ggcaaaaagc
      421 cggtcttgca gaattttttc agcacgcagg ctgtctcggc ggtgcaccaa ggtgacatgc
      481 gcggcatggt gggtgagata cagggcctct tcaaccgcag tattaccgcc gccgacaacc
      541 acaacgttct tcccgcggta gaaaaagcca tcgcaggtgg cgctagcaga aacaccggca
      601 ccttgcagat gcttttcact tggcaggcca agccagcgtg cctgtgcgcc tgtggcaaca
      661 ataacagcac gggccttgta ggttgtgccg gaatcacctg ttagcagaaa aggtgaaccc
      721 gctttgaaat taacagatgt aataatgtcg tattcaatgc gtgtgcccac gttttgcgcc
      781 tgggctgcca tttgttccat cagccacggc ccctgtatgg ctgtggcaaa acccggatag
      841 ttttcaacat ctgtagtaat ggtgagctgc ccgccaggct gaaggccagc gaccagaacg
      901 ggagaaaggt tggcacgtgc cgcataaata gctgcggtgt atccggcagg tcccgcacca
      961 ataacgagca gatcagttgt aatggtttca gccatatgta tatctccttc ttaaagttaa
     1021 acaaaattat ttctagaggg aaaccgttgt ggtctcccta tagtgagtcg tattaatttc
     1081 gcgggatcga gatctcgggc a
//
