LOCUS       sequence-134549-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express copGFP when FLP is expressed by another vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-Fon-copGFP
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..60
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..757
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
ORIGIN
        1 atccagaggt tgattgtcga cgaattcgaa gttcctattc tctgaaagta taggaacttc
       61 gctagccacc atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat
      121 caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa
      181 gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta
      241 cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta
      301 cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa
      361 gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt
      421 gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga
      481 caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct
      541 ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt
      601 ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc
      661 catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga
      721 gtaccagcac gccttcaaga cccccattgc cttcgccaga tcccgcgctc agtcgtccaa
      781 ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgctaag gcgcgccga
//
