LOCUS       sequence-134717-        8682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EXPRESs plasmid for human erythrocyte surface proteins encoding Kell
            blood group with bio-rat CD4d3+4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, 
            Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner 
            JC, Wright GJ
  TITLE     Basigin is a receptor essential for erythrocyte invasion by 
            Plasmodium falciparum.
  JOURNAL   Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606.
  PUBMED    22080952
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-11-9- 9"; volume: "480"; issue: "7378"; pages:
            "534-7"
COMMENT     Sequence Label: bio-CD4d3+4-Kell blood group
FEATURES             Location/Qualifiers
     source          1..8682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2782..2837
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2836..2855)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5073..5091)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5159..5263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5264..5332,5333..6124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5482..5501)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6295..6883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6784..6803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7037..7054
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7171..7192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7207..7212,7213..7230,7231..7237)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7245..7261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7250..7272
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7269..7285
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7269..7285
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7556..7935
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7936..8139
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8090..8110
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8136..8160
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     8641..8660
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtgcc
       61 tgtgcagaca ttgtgggtcc agacctgcat catattctgg atgcacagaa aatggtgtgg
      121 aatcatcgtg acagaaacct gcctccacta gcacctctgt cgacctccat cacggcctat
      181 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
      241 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
      301 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
      361 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
      421 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
      481 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
      541 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
      601 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
      661 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttcgcggccg
      721 cccttccaga attgcggccc cagaccctgc gagacaagcg tgtgcctgga tctgcgggat
      781 cactatctgg ccagcggcaa cacaagcgtg gccccctgca ccgatttctt cagcttcgcc
      841 tgcggcagag ccaaagagac aaacaacagc ttccaggaac tggccaccaa gaacaagaac
      901 cggctgcggc ggatcctgga agtgcagaac agctggcacc ctggcagcgg cgaggaaaag
      961 gccttccagt tctacaacag ctgcatggac accctggcca tcgaagccgc cggaacaggg
     1021 cctctgcgcc aggtcatcga ggaactgggc ggctggcgga tcagcggcaa gtggaccagc
     1081 ctgaacttca accggaccct gagactgctg atgagccagt acggccactt ccctttcttc
     1141 cgggcctacc tgggacctca ccctgccagc cctcacaccc ccgtgatcca gatcgaccag
     1201 cccgagttcg acgtgcccct gaagcaggac caggaacaga agatctacgc ccagatcttt
     1261 agagaatatc tcacatatct gaaccagctg ggaacactcc tgggaggcga cccttctaaa
     1321 gtccaggaac atagcagcct gagcatcagc atcacctcta gactcttcca gttcctgcgg
     1381 cccctggaac agagaagggc ccagggcaag ctgttccaga tggtcacaat cgatcagctc
     1441 aaagagatgg cccctgccat cgactggctg agttgtctcc aggccacctt cacccccatg
     1501 agcctgagcc ctagccagag cctggtggtg cacgacgtgg agtatctgaa gaacatgagc
     1561 cagctggtcg aggaaatgct gctgaagcag cgggacttcc tgcagagcca catgatcctg
     1621 ggcctggtgg tcacactgag ccctgccctg gactctcagt tccaggaagc cagacggaag
     1681 ctgtcccaga agctgcggga gctgaccgag cagcctccca tgcccgccag accccggtgg
     1741 atgaagtgcg tggaggaaac cggcaccttc ttcgagccta ccctggccgc cctgttcgtg
     1801 agagaggcct tcggccccag cacaagatcc gccgccatga agctgttcac cgccatccgg
     1861 gacgccctga tcacccggct gagaaatctg ccttggatga acgaagaaac ccagaacatg
     1921 gcccaggaca aggtggcaca gctccaggtc gagatgggag cctcagagtg ggccctgaag
     1981 cccgaactgg cccggcagga atacaacgac atccagctgg gctccagctt cctgcagtcc
     2041 gtgctgtcct gcgtgagaag cctgcgggcc agaatcgtgc agtccttcct gcagcctcat
     2101 ccacagcata gatggaaggt gtccccctgg gacgtgaacg cctactacag cgtgtccgac
     2161 cacgtggtgg tgtttccagc cggcctgctg cagcctccct tcttccaccc tggatatcct
     2221 cgcgctgtga actttggcgc cgctggcagc atcatggccc acgagctgct gcacatcttc
     2281 taccagctgc tgctgcctgg cggatgcctg gcctgcgaca accatgctct ccaggaagcc
     2341 cacctctgcc tgaagagaca ctacgccgcc ttccctctgc ctagccggac cagcttcaac
     2401 gacagcctga ccttcctgga aaacgccgcc gatgtgggcg gactggccat tgctctgcag
     2461 gcctacagca aacgcctcct cagacaccac ggcgagacag tgctgccctc cctggacctg
     2521 agcccccagc agatcttctt cagatcctat gcccaggtca tgtgcagaaa gcccagcccc
     2581 caggacagcc acgataccca cagcccccct cacctgagag tgcacggccc tctgagcagc
     2641 acacccgcct tcgcccggta cttcagatgt gccagaggcg ccctgctgaa cccctctagc
     2701 cgatgtcagc tctggtgagg cgcgcctgaa ttcttctaga cacgtgatta attaaggatc
     2761 ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     2821 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc
     2881 ctcggagatc tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga
     2941 catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact
     3001 tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc
     3061 tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc
     3121 atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact
     3181 cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga
     3241 ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc
     3301 ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc
     3361 atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt
     3421 tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc
     3481 gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag
     3541 ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac
     3601 tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg
     3661 agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc
     3721 agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc
     3781 tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa
     3841 caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat
     3901 ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg
     3961 tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga
     4021 aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt
     4081 aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg
     4141 aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta
     4201 aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact
     4261 tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca
     4321 agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc
     4381 caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg
     4441 ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     4501 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     4561 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     4621 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
     4681 tccgggtagc atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata
     4741 tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4801 ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     4861 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     4921 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4981 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg
     5041 tcaaacatga gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     5101 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5161 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5221 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5281 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5341 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5401 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5461 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     5521 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5581 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5641 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5701 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5761 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     5821 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5881 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5941 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6001 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6061 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6121 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6181 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6241 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6421 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6841 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6901 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6961 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7021 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     7081 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7141 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7201 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7261 atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca
     7321 attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag
     7381 gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca attagccata
     7441 ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
     7501 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat
     7561 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     7621 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     7681 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     7741 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     7801 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     7861 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
     7921 atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt
     7981 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     8041 caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc
     8101 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     8161 ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tgggctcgcg gttgaggaca
     8221 aactcttcgc ggtctttcca gtactcttgg atcggaaacc cgtcggcctc cgaacggtac
     8281 tccgccaccg agggacctga gcgagtccgc atcgaccgga tcggaaaacc tctcgagaaa
     8341 ggcgtctaac cagtcacagt cgcaaggtag gctgagcacc gtggcgggcg gcagcgggtg
     8401 gcggtcgggg ttgtttctgg cggaggtgct gctgatgatg taattaaagt aggcggtctt
     8461 gagacggcgg atggtcgagg tgaggtgtgg caggcttgag atccagctgt tggggtgagt
     8521 actccctctc aaaagcgggc attacttctg cgctaagatt gtcagtttcc aaaaacgagg
     8581 aggatttgat attcacctgg cccgatctgg ccatacactt gagtgacaat gacatccact
     8641 ttgcctttct ctccacaggt gtccactccc aggtccaagt tt
//
