LOCUS       sequence-134718-        7848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EXPRESs plasmid for human erythrocyte surface proteins encoding ADL1
            with bio-rat CD4d3+4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, 
            Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner 
            JC, Wright GJ
  TITLE     Basigin is a receptor essential for erythrocyte invasion by 
            Plasmodium falciparum.
  JOURNAL   Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606.
  PUBMED    22080952
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-11-9- 9"; volume: "480"; issue: "7378"; pages:
            "534-7"
COMMENT     Sequence Label: bio-CD4d3+4-ADL1
FEATURES             Location/Qualifiers
     source          1..7848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1948..2003
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2002..2021)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4239..4257)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4325..4429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4430..4498,4499..5290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4648..4667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5461..6049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5950..5969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6203..6220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6337..6358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6373..6378,6379..6396,6397..6403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6411..6427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6416..6438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6435..6451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6435..6451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6722..7101
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7102..7305
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7256..7276
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7302..7326
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     7807..7826
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtgcc
       61 tgtgcagaca ttgtgggtcc agacctgcat catattctgg atgcacagaa aatggtgtgg
      121 aatcatcgtg acagaaacct gcctccacta gcacctctgt cgacctccat cacggcctat
      181 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
      241 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
      301 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
      361 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
      421 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
      481 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
      541 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
      601 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
      661 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttcgcggccg
      721 cctctgcctg gcagcgtgtc cgacccctgg aagctgatgc tgctggacgc tacctttaga
      781 ggcgcccagc aagtcagcaa cctgatccac tacctgggcc tgagccacca tctgctggcc
      841 ctgaacttca tcatcgtgtc cttcggcaag aagtccgcct ggtccagcgc ccaggtcaaa
      901 gtgaccgaca ccgacttcga cggcgtggaa gtgcgcgtgt tcgagggacc tcccaagccc
      961 gaggaacccc tgaagcggag cgtggtgtac atccacggcg gaggatgggc tctggctagc
     1021 gccaagatcc ggtactacga cgagctgtgc accgccatgg ccgaggaact gaacgccgtg
     1081 atcgtgtcca tcgagtaccg gctggtgccc aaggtgtact tccccgagca gatccacgat
     1141 gtcgtgcggg ccacaaagta ctttctgaag cccgaggtgc tgcagaaata catggtggac
     1201 cctggcagaa tctgcatcag cggcgacagc gctggcggaa atctggccgc tgccctgggc
     1261 cagcagttca cccaggacgc cagcctgaag aacaagctga agctgcaggc cctgatctac
     1321 cccgtgctgc aggctctgga cttcaacacc cccagctacc agcagaacgt gaacaccccc
     1381 atcctgccca gatacgtgat ggtcaagtac tgggtggact acttcaaggg caactacgac
     1441 ttcgtgcagg ccatgatcgt gaacaaccac accagcctgg acgtggaaga ggccgctgcc
     1501 gtgagagcca gactgaactg gaccagcctg ctgcccgcca gcttcaccaa gaactacaag
     1561 cccgtggtgc agaccaccgg caacgcccgg atcgtgcagg aactgcccca gctgctggat
     1621 gccagaagcg cccctctgat tgccgatcag gctgtgctgc agctgctgcc caagacctac
     1681 atcctgacat gcgagcacga cgtgctgcgg gacgacggca tcatgtacgc caagcggctg
     1741 gaatctgccg gggtggaagt gaccctggac cacttcgagg acggcttcca cggctgcatg
     1801 atcttcacca gctggcccac caacttcagc gtgggcatcc ggacccggaa cagctacatc
     1861 aagtggctgg accagaatct gtgaggcgcg cctgaattct tctagacacg tgattaatta
     1921 aggatccccc gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     1981 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg
     2041 agatccctcg gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga
     2101 ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt
     2161 acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg
     2221 gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg
     2281 gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt
     2341 gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca
     2401 ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag
     2461 cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg
     2521 ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta attcaatagc
     2581 atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg
     2641 aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc
     2701 cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca
     2761 gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa
     2821 ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac
     2881 gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata
     2941 taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat
     3001 ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac
     3061 acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa
     3121 gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca
     3181 aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg
     3241 aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt
     3301 tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg
     3361 actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa
     3421 accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc
     3481 gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct
     3541 gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg
     3601 ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa
     3661 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     3721 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa
     3781 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     3841 tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa
     3901 tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     3961 tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4021 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4081 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4141 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat
     4201 aagctgtcaa acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt
     4261 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4321 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4381 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4441 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4501 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4561 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4621 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     4681 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4741 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4801 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4861 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4921 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     4981 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5041 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5101 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5161 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5221 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5281 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5341 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5461 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5521 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5581 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5641 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5701 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5761 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5821 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5881 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5941 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6001 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6061 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     6241 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     6301 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     6361 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     6421 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     6481 cgaccaattc tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg
     6541 ctgcaggcgc agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta
     6601 gccatattag tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg
     6661 ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg accgccatgt
     6721 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     6781 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     6841 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     6901 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     6961 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
     7021 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
     7081 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
     7141 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     7201 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
     7261 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     7321 cagatcctca ctctcttccg catcgctgtc tgcgagggcc agctgttggg ctcgcggttg
     7381 aggacaaact cttcgcggtc tttccagtac tcttggatcg gaaacccgtc ggcctccgaa
     7441 cggtactccg ccaccgaggg acctgagcga gtccgcatcg accggatcgg aaaacctctc
     7501 gagaaaggcg tctaaccagt cacagtcgca aggtaggctg agcaccgtgg cgggcggcag
     7561 cgggtggcgg tcggggttgt ttctggcgga ggtgctgctg atgatgtaat taaagtaggc
     7621 ggtcttgaga cggcggatgg tcgaggtgag gtgtggcagg cttgagatcc agctgttggg
     7681 gtgagtactc cctctcaaaa gcgggcatta cttctgcgct aagattgtca gtttccaaaa
     7741 acgaggagga tttgatattc acctggcccg atctggccat acacttgagt gacaatgaca
     7801 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaagttt
//
