LOCUS       sequence-134745-         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RHOA
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ctgccatccg gaagaaactg gtgattgttg gtgatggagc ctgtggaaag
      121 acatgcttgc tcatagtctt cagcaaggac cagttcccag aggtgtatgt gcccacagtg
      181 tttgagaact atgtggcaga tatcgaggtg gatggaaagc aggtagaatt ggctttgtgg
      241 gacacagctg ggcaggaaga ttatgatcgc ctgaggcccc tctcctaccc agataccgat
      301 gttatactga tgtgtttttc catcgacagc cctgatagtt tagaaaacat cccagaaaag
      361 tggaccccag aagtcaagca tttctgtccc aacgtgccca tcatcctggt tgggaataag
      421 aaggatcttc ggaatgatga gcacacaagg cgggagctag ccaagatgaa gcaggagccg
      481 gtgaaacctg aagaaggcag agatatggca aacaggattg gcgcttttgg gtacatggag
      541 tgttcagcaa agaccaaaga tggagtgaga gaggtttttg aaatggctac gagagctgct
      601 ctgcaagcta gacgtgggtg acagtaaagg tggatacgga tccgaa
//
