LOCUS       sequence-134753-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPIN4
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga ctcgtaggaa catcgtgggc tgccgcattc aacacggctg gaaggaaggc
      121 aacgagccag tggagcagtg gaagggtact gtgctcgagc aggtttccgt gaagcccact
      181 ctttacatca ttaaatatga tggcaaagat agtgtgtatg gactagaact gcaccgcgat
      241 aagagagttt tagcgctaga gatccttcct gagagagtgc caactcctcg tatcgattca
      301 cgactggcag attccctgat tggcaaggca gtggagcatg tgtttgaagg tgaacatggt
      361 accaaggatg aatggaaggg tatggtcctg gcgcgagctc ctgtgatgga tacttggttt
      421 tacatcacct acgagaaaga tcctgttctc tatatgtaca cgctgcttga tgactacaaa
      481 gatggtgact tacgcattat tccagattcc aactactatt tccctacagc agaacaggag
      541 cctggagagg tggtcgacag tctcgtgggc aagcaggtgg agcatgccaa agatgacggg
      601 tccaagagaa ctggcatttt tatacatcaa gtggtggcga agccatctgt ttacttcatt
      661 aagtttgatg atgatattca catttatgtc tatggtttgg tgaaaactcc ttgacagtaa
      721 aggtggatac ggatccgaa
//
