LOCUS       sequence-134763-         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SHC1
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gccagcttgg gggcgaggag tggacccgcc acgggagctt tgtcaataag
      121 cccacgcggg gctggctgca tcccaacgac aaagtcatgg gacccggggt ttcctacttg
      181 gttcggtaca tgggttgtgt ggaggtcctc cagtcaatgc gtgccctgga cttcaacacc
      241 cggactcagg tcaccaggga ggccatcagt ctggtgtgtg aggctgtgcc gggtgctaag
      301 ggggcgacaa ggaggagaaa gccctgtagc cgcccgctca gctctatcct ggggaggagt
      361 aacctgaaat ttgctggaat gccaatcact ctcaccgtct ccaccagcag cctcaacctc
      421 atggccgcag actgcaaaca gatcatcgcc aaccaccaca tgcaatctat ctcatttgca
      481 tccggcgggg atccggacac agccgagtat gtcgcctatg ttgccaaaga ccctgtgaat
      541 cagagagcct gccacattct ggagtgtccc gaagggcttg cccaggatgt catcagcacc
      601 attggccagg ccttcgagtt gcgctgacag taaaggtgga tacggatccg aa
//
