LOCUS       sequence-134765-        1241 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1241)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BMP2K
FEATURES             Location/Qualifiers
     source          1..1241
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..201
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             208..228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1221..1238
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtggataaca agttcaacaa ggagcgtcga
       61 agagctcgcc gtgaaattcg ccatctgccg aacctgaacc gcgaacagcg tcgcgcattt
      121 attcgcagcc tgcgcgatga tccgagccag agcgcgaacc tgctggcgga agcgaagaag
      181 ctgaacgatg cgcagccgaa gggtaccgag aacctgtact tccaatccat gtccgtgggg
      241 gtccgggtgt tcgcggtcgg ccgccaccag gtcaccctgg aagagtcgct ggccgaaggt
      301 ggattctcca cagttttcct cgtgcgtact cacggtggaa tccgatgtgc attgaagcga
      361 atgtatgtca ataacatgcc agacctcaat gtttgtaaaa gggaaattac aattatgaaa
      421 gagctatctg gtcacaaaaa tattgtgggc tatttggact gtgctgttaa ttcaattagt
      481 gataatgtat gggaagtcct tatcttaatg gaatattgtc gagctggaca ggtagtgaat
      541 caaatgaata agaagctaca gacgggtttt acagaaccag aagtgttaca gatattctgt
      601 gatacctgtg aagctgttgc aaggttgcat cagtgtaaga ctccaataat tcaccgggat
      661 ctgaaggtag aaaatatttt gttgaatgat ggtgggaact atgtactttg tgactttggc
      721 agtgccacta ataaatttct taatcctcaa aaagatggag ttaatgtagt agaagaagaa
      781 attaaaaagt atacaactct gtcatacaga gcccctgaaa tgatcaacct ttatggaggg
      841 aaacccatca ccaccaaggc tgatatctgg gcactgggat gtctactcta taaactttgt
      901 ttcttcactc ttccttttgg tgagagtcag gttgctatct gtgatggcaa cttcaccatc
      961 ccagacaatt ctcgttactc ccgtaacata cattgcttaa taaggttcat gcttgaacca
     1021 gatccggaac atagacctga tatatttcaa gtgtcatatt ttgcatttaa atttgccgcg
     1081 gctgattgtc cagtctccaa catcaataat tcttctattc cttcagctct tcctgaaccg
     1141 atgactgcta gtgaatgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
     1201 agcttgcggc cgcactcgag caccaccacc accaccactg a
//
