LOCUS       sequence-134803-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression, codon optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-HA-Sestrin2
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ataagctgca taacagttgg gccatggcgg ccaagctggg gatccttact tacttagcgg
       61 ccgctcaggt catgtagcgg gtgatggcac ggagggcgta cagcagagcg gcttgcatgc
      121 gcgcctccag gagcagcaag ttcacgtgga ccttctctga gtggcggaag tgcctccaga
      181 agaggttgta cattcttcgg gtggtcttct ctgggtagca ggccactgtc ttgatataga
      241 ccttgaggtt ccgctccagg agctggttca cctccccata atcatagtca tcatatctga
      301 tgccaaagac gcagtggata tagttccaga tggccctgcg gagcacggag gtgtccacac
      361 cactgtgcat ggcgatggta ttgtaggtga ggctataggc tgcctggaac ttctcatcca
      421 gcagctgccc accctcaggg taaagccgct ggatcagcga gtagccatgg tcttcccagg
      481 tataatcctg ggcccggaag gtagggggtg cctgagcccc tctccgagtg aagtcctcat
      541 atccgaaagt agggtcttcc acaaagcaca gcatgtctgg gtgtggagag ggctccagga
      601 tgtcagctga gggggtcacc agcaggctct ctgacttctc cagctcaaag cggctctcca
      661 tctcctcctg ggacgtcccc tcatcccgca gcaggctctc ctgcagctgc tgcatgcgct
      721 ccatcagcgc ctccacgtcg cgggcagact caaagccccc agagttgttc aacgggtccc
      781 tgcttggggg gctgctctgt tcactagggg gtgtaggtgc ctggggggca gggctgccat
      841 ctgcatcccc ctcagggagg atgccacagc caaac
//
