LOCUS       sequence-134896-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modular shuttle vector for Geobacillus and E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Reeve B, Martinez-Klimova E, de Jonghe J, Leak DJ, Ellis T
  TITLE     The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile 
            Engineering.
  JOURNAL   ACS Synth Biol. 2016 Jul 1.
  PUBMED    27332993
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jul 1."
COMMENT     Sequence Label: pG1AK-mCherry
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(293..309)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(293..309)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(306..328)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    317..333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(341..347,348..365,366..371))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    386..407
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(524..541)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
       61 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      121 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      181 gaacagtacg aacgcgccga gggccgccac tccacctaat aagagctcaa tttcgcgccc
      241 cgaaaggggc gtttttttgc gaagcctaag gtatcattta aatgtaatca tggtcatagc
      301 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      361 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      421 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      481 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      541 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      601 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      661 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccc
//
