LOCUS       sequence-134907-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human APE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Schuermann D, Scheidegger SP, Weber AR, Bjoras M, Leumann CJ, Schar 
            P
  TITLE     3CAPS - a structural AP-site analogue as a tool to investigate DNA 
            base excision repair.
  JOURNAL   Nucleic Acids Res. 2016 Jan 4. pii: gkv1520.
  PUBMED    26733580
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jan 4. pii: gkv1520."
COMMENT     Sequence Label: pET28HIS-hAPE1
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    59..83
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             98..120
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg
       61 aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga
      121 tataccatgg gatccatgcc gaagcgtggg aaaaagggag cggtggcgga agacggggat
      181 gagctcagga cagagccaga ggccaagaag agtaagacgg ccgcaaagaa aaatgacaaa
      241 gaggcagcag gagagggccc agccctgtat gaggaccccc cagatcagaa aacctcaccc
      301 agtggcaaac ctgccacact caagatctgc tcttggaatg tggatgggct tcgagcctgg
      361 attaagaaga aaggattaga ttgggtaaag gaagaagccc cagatatact gtgccttcaa
      421 gagaccaaat gttcagagaa caaactacca gctgaacttc aggagctgcc tggactctct
      481 catcaatact ggtcagctcc ttcggacaag gaagggtaca gtggcgtggg cctgctttcc
      541 cgccagtgcc cactcaaagt ttcttacggc ataggcgatg aggagcatga tcaggaaggc
      601 cgggtgattg tggctgaatt tgactcgttt gtgctggtaa cagcatatgt acctaatgca
      661 ggccgaggtc tggtacgact ggagtaccgg cagcgctggg atgaagcctt tcgcaagttc
      721 ctgaagggcc tggcttcccg aaagcccctt gtgctgtgtg gagacctcaa tgtggcacat
      781 gaagaa
//
