LOCUS       sequence-135011-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LseC2c2 locus with one spacer in array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, 
            Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, 
            Koonin EV
  TITLE     Discovery and Functional Characterization of Diverse Class 2 
            CRISPR-Cas Systems.
  JOURNAL   Mol Cell. 2015 Nov 5;60(3):385-97. doi: 
            10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
  PUBMED    26593719
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.008"; journalName: "Mol Cell"; date: 
            "2015-11-5- 5"; volume: "60"; issue: "3"; pages: "385-97"
COMMENT     Sequence Label: pZ004 (LseC2c2 locus)
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttac tttagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gtgccgcgcg gcagccatat ggctagctac tgtgtgcata agtcttcctt
      121 agatccatag gtacagcagt tttatttatt agccttagaa aatggaaaat agagcttata
      181 aatgatatga tatttatgaa taaaatgatt gcattctcgt gcaaacttta aatatattga
      241 ttatatcctt tacattggtt gttttaatta ctattattaa gtaggaatac gatatacctc
      301 taaatgaaag aggactaaaa cccgccaaaa gtatcagaaa atgttattgc agtaagagac
      361 tacctctata tgaaagagga ctaaaacttt taacagtggc cttattaaat gacttctgta
      421 agagactacc tctatatgaa agaggactaa aacgtctaat gtggataagt ataaaaacgc
      481 ttatccatca tttaggtgtt ttattttttt gtgattatat gtacaataga agagagaaaa
      541 aaatcattga ggtgaaaact atgagaatta ctaaagtaga ggttgataga aaaaaagtac
      601 taatttctag ggataaaaac gggggcaagt tagtttatga aaatgaaatg caagataata
      661 cagaacaaat catgcatcac aaaaaaagtt ctttttacaa aagtgtggta aacaaaacta
      721 tttgtcgtcc tgaacaaaaa caaatgaaaa aattagttca tggattatta caagaaaata
      781 gtcaagaaaa aataaaagtt tcagatgtca ctaaacttaa tatctcaaat ttcttaaatc
      841 atcgtttca
//
