LOCUS       sequence-135104-       14590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  unc-70 no-force control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14590)
  AUTHORS   Krieg M, Dunn AR, Goodman MB
  TITLE     Mechanical control of the sense of touch by beta-spectrin.
  JOURNAL   Nat Cell Biol. 2014 Mar;16(3):224-33. doi: 10.1038/ncb2915. Epub 
            2014 Feb 23.
  PUBMED    24561618
REFERENCE   2  (bases 1 to 14590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2915"; 
            journalName: "Nat Cell Biol"; date: "2014-03"; volume: "16"; issue: 
            "3"; pages: "224-33"
COMMENT     Sequence Label: mg409
FEATURES             Location/Qualifiers
     source          1..14590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2379..3086
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(3207..3209,3210..3212,3213..3923)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3252..3273)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3513..3532)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3860..3881
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(11478..11497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11479..11497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11504..11521)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11504..11520)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11513..11535)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             11658..11960
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11839..11858
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             12309..13103
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             12309..13103
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12363..12382)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12973..12992
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      13303..13312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13309..13683
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      13821..14409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14310..14329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14563..14580
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgacg cgttagaata ctcaagctat gcatcaagct tatttttaat gctactcatt
      301 aagaacttac ttgacattag aactcaaaat ctaattgttg ttcggaatta atttcgaata
      361 catcaattta tcttattctc tcctcccgca atcataaacc accagaccag tagcatctgt
      421 aaagtgcgcg gccttcctcc acccatcact ctctcgtaac ctcaatgaaa tagtggcgcg
      481 ttcgatgagg gtatcctcct gcgtctgtgg tacacaaaac gctatttttt gcctgcaacc
      541 cgggggccct ctttctctct cggtaccctc cacccaatcg atcggatgtg tgccaaccct
      601 tcttgcctgc atcttttgcg cgcaccaacg gtcctctctg ccgctctctc tgactctccg
      661 acaatgctgc actctacgtt ctccacatca ttccgactcc gtctctccgt cgtcgcatcc
      721 gtccccgtcg tttagccaat cctccttttc ctcttcctcc tcctcgtttt cttctcgatt
      781 tcaattccag cagcagcagc actgtcgtcg cttttcgttc taggatctct acttctttat
      841 tgtgatattg ttttcttgtt gttcgcggtg agatagtaaa gaaaaagaga gaggcgccgc
      901 gcatcccgtt cgtcaaaacg atgttgccgt tacccaatat aacgattgtt gtcttttgcc
      961 attttgtagg aactgtcgag ttgtaaacaa catgcaaaat ttggaacatt ttttgaactg
     1021 aggttcgcat ttcgttgttt atgaatcaaa atgttacttt ttttcggtga gaacggagat
     1081 ttgcagttca cctggaacta ggttcttttt atttttattt tgtctacaaa ctgaatgttg
     1141 tttagttttg ttatcgataa caatgcaatt ctcaaatttg aaattgtgga aacaactgca
     1201 aggtgccaga agttttgaat ctgaaaccac gacattatct ttttgaaact atttttttga
     1261 atgaatattt gcagttctaa aaagtaaatc gtcatttcaa aattgtttct catgcaaaaa
     1321 taaaggcaag atcaaatgtg tttttgaata gacctgcgtt tttcagatca gtagatcaat
     1381 cagcgtccct agaaatattc tcaaaatttt tctcgtgcca actcaaacat ttgctcccat
     1441 attttcgtaa tttcttatag tatgcttttt tcgtcgtctt ctcctgctac atgtattcag
     1501 tgctaatcct aatcccttct gctaatttta tccgctttcc catccacatc cacacacatt
     1561 ctttttctct ttttattggc tgctcccact cgacactcat ttgggccaca aggacttggg
     1621 ggacccatgt gtatgttcga gttgtggggc tctatgtgtg tgtgtgtgtg tgtgtgtttg
     1681 agtgcgcctc tacgcagttg tcgagggccc cagccggcgg tctcccccaa cggccactgc
     1741 cctgcttcta cgtctttttc ttcttgtcct ccacctcccc aaccactggg tgcccttgct
     1801 tttgaaccaa acgggcagtg ctcccttttc cttccactta tttgattcgt cccaatgttt
     1861 gtgtctctca ccctctcaat cttttcatta cttgaataca ttgctttcta aactttttct
     1921 tccttctttt ctgattcttc ttgttttcca cccttttcca attggcagag catgggagca
     1981 aagggccctc ctactaattg ccaatgctcc gttgttccac catttttttt gtgttgtttt
     2041 ttgtcgtctc ctctccatcc agccgtcgtg tatttttctc tacgcctcat gaactatttt
     2101 catcaactcg ccaattcata agcccccttg gcttaaatga atgcgagggt ccctttttct
     2161 gtcaattttt tttctcgcct ctgctcagca acactggaaa tcgagacagg tttttattaa
     2221 tctatgattt ttcagcaaaa tttcggcggg acttgcaacg gcgcgaaacg tcgtcggcaa
     2281 tatactagta acggccgcca gtgtgctgga attcaggcaa aatttcggcg ggacttgcaa
     2341 cggcgcgaaa cgtcgtcggc aatatggcta cggtcgacat ggtgagcaag ggcgaggaga
     2401 ccacaatggg cgtaatcaag cccgacatga agatcaagct gaagatggag ggcaacgtga
     2461 atggccacgc cttcgtgatc gagggcgagg gcgagggcaa gccctacgac ggcaccaaca
     2521 ccatcaacct ggaggtgaag gagggagccc ccctgccctt ctcctacgac attctgacca
     2581 ccgcgttcgc ctacggcaac agggccttca ccaagtaccc cgacgacatc cccaactact
     2641 tcaagcagtc cttccccgag ggctactctt gggagcgcac catgaccttc gaggacaagg
     2701 gcatcgtgaa ggtgaagtcc gacatctcca tggaggagga ctccttcatc tacgagatac
     2761 acctcaaggg cgagaacttc ccccccaacg gccccgtgat gcagaagaag accaccggct
     2821 gggacgcctc caccgagagg atgtacgtgc gcgacggcgt gctgaagggc gacgtcaagc
     2881 acaagctgct gctggagggc ggcggccacc accgcgttga cttcaagacc atctacaggg
     2941 ccaagaaggc ggtgaagctg cccgactatc actttgtgga ccaccgcatc gagatcctga
     3001 accacgacaa ggactacaac aaggtgaccg tttacgagag cgccgtggcc cgcaactcca
     3061 ccgacggcat ggacgagctg tacaaggggc caggtggtgc agggccaggt ggtgcagggc
     3121 caggtggtgc agggccaggt ggtgcagggc ccggtggtgc aggtccaggt ggtgcaggtc
     3181 caggtggtgc aggtccaggt ggtgctatgg tgagcaaggg cgaggagctg ttcaccgggg
     3241 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3301 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
     3361 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct
     3421 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     3481 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     3541 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     3601 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     3661 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     3721 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     3781 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg agcaaagacc
     3841 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     3901 tcggcatgga cgagctgtac aagggaggag gagtcgacgg caattcaggc gcctataccc
     3961 aagatgagga ctatgatgac aactcgtccg cccgcctctt cgaacgttct cgtatcaagg
     4021 cactagcaga tgaacgtgaa ctcgtgcaaa agaaaacatt tacaaaatgg gtcaactcac
     4081 acttggttcg agtcagctgc aaagtacaag acctctacat ggatatgcga gacggaaaaa
     4141 tgctcctccg actcctcgca gttctttccg gtgaacgtct gccgaaaccg acgccgggaa
     4201 aaatgcgtat ccactgtctt gagaacgttg agaaaggttt gcaattcttg cgcaatcaac
     4261 acgtgcatct cgaaaacttg ggttcacacg atatcgttga cggaaattca cgcctcacgt
     4321 tgggtctcat ctggacgatc atccttcgat tccagatcca agatatcaca tttgaagacg
     4381 ctgacaatca cgagacacgt tcagcgaagg aggccttgtt actgtggtgc cagatgaaaa
     4441 ctgctggata tccaaatgtc aacgtcaaga acttctcaac cagttggaga gatggtcttg
     4501 cattcaatgc acttattcat aaacatagac ctgatctcgt agactacgat aatttacaaa
     4561 aatcgaatgc tctttacaat ttgcaatctg cttttgatac cgcagaaaat caattgggat
     4621 tggccaagtt cctcgatgcc gaagatgtga acgttgacca acctgatgag aaatccatta
     4681 tcacttatgt agttacatat tatcattact tcaacaaact gaaacaagat aacatccaag
     4741 gaaaacgtat cggaaaagtg attaatgagt tgatggagaa cgacaaaatg atcaatcgat
     4801 acgaaactct ttcatctgat ttgctcgaat ggatcaatgc caagatccaa cttctcaacg
     4861 aacgtcattt tgagaataat ttggaaggtg ttcaaagaca gctcaccgag ttcaacgact
     4921 accgtactca ggagaaacca ccaaagttcg atgaaaaggg agagctcgaa gtacttctgt
     4981 tcactcttca atcagctatg cgtgccaaca atcagagacc tttcgtgccg agagaaggaa
     5041 aactgattgc tgatatcaac cgagcatggc aatcccttga gaaggcagaa cacgaacgag
     5101 aacttgtcct gaaagaggaa ctcattcgtc aagagaaact cgaacaactc gcagcccgat
     5161 tcaacagaaa ggccgagatg cgagagactt ggctcacaga aaaccaacgg cttgtcagtc
     5221 aggacaattt cggaaacgac ttgtcttcgg tggaagctgc caccaaaaag cacgaagcca
     5281 tcgagacgga catctttgct tacgaggagc gtgttcaagc cgtcgtcgct gttgctggag
     5341 agcttgaagc cgagaactac catgatcaag ccaaaatcaa tgagagaaag gagaatgttc
     5401 ttcaactctg gaactatctt ttccaacttc ttttggctcg cagagttcga ttggagttgt
     5461 caatggccat tcaaaagatc ttccacgata tgcttcttac tctcgatctc atggatgata
     5521 ttaagagcag acttttaagc gaggatcttg gagcccatct tatggacgta gaagatttgc
     5581 ttcaaaagca cgctcttctt gagtctgata ttaatattat tggagaacga gttaacaatt
     5641 ctattgctca agctcaacgc ttccgaaatc cagatggacc agatggaagt ggatacaagc
     5701 cagttgaacc aggaaccatt gatgagagaa gtgatgttct acaaaaacgt tacaaggagc
     5761 ttcttgattt ggctgctgaa cgtaagcgtc gtctcgaaga caacaagaga ctttgccagt
     5821 tctggtggga tgttgctgag cttgaacatg gtattaagga acaagagcaa gttctttcgt
     5881 ccactgatac tggccgtgat attgtcactg tttcgcattt gttggccaaa cataagaacg
     5941 ctgagaacaa ccttcgcgac ttggagaagt acctcgatcg tcttgatgtt tctggaaaag
     6001 aattgcaaga tgaaagcatc ccaggaagtg acaacattcc tccaaggctt gccgagattc
     6061 gtgattacat caacaagctc aaggaactct ctgcttctcg caaagaaaga cttgccggtg
     6121 gagtcgaata ctaccaattc ttcaccgacg ccgacgatgt cgatcgctac ttgtatgata
     6181 ctctccgcgt catgtcatcc gaagacgttg gaaaagacga gggaactgtt caacttctcc
     6241 tcaagaagca cgacgatgtc catgacgagc tccagaactt tgaccaacac atcaaggttc
     6301 ttcacgccaa ggccgagtct cttccacagg aagccagaga acatccagat atcagacaac
     6361 gattggacac aactctcaag cagaaggccg aactcgaaaa cctttctcaa cttcgcaagc
     6421 agagacttat cgatgctctc tccctgtaca agttgtactc ggatgccgat tcagtcgagt
     6481 cttggatcga tgagaaggga aaacttttgg caacattggt ccctggacgc gatatcgaag
     6541 aggttgagat catgaagcat cgtttcgata ctcttgagca agatatgaag aatcaagaag
     6601 ctaaggttac taacgtcaat gacttggctc gtcaattgct taatgtggaa catccaaact
     6661 ccgacgacat tcttcaccgt caaaacaagc tcaacgctcg ctgggctcaa cttcgcgaca
     6721 tggtcgatca aaagagaaac gagctcgaga gagctcaccg cctcgaaact ttccgcatcg
     6781 attgccaaga aacagttact tggatcgagg acaagactcg tgtactcgag gattcggacg
     6841 cgttgaccaa cgatctttcc ggtgtcatga aacttcaaag aagattgtcc atgatggaac
     6901 gcgatttggg agcgattcaa gccaagctcg actcgcttca caaagaagcc gatgacatcg
     6961 aaagagaacg accacaagaa gctcaagcca ttcgtgaaga tatcaagaga atccaccaag
     7021 tctgggatat ccttaacaag aaagttcgtg agcacgaagc taaactcgat gaagctggag
     7081 atcttcaacg cttcctccgt gatttggatc acttccaagc ctggcttact gccacccaga
     7141 gacaagttgc ctcagaggaa gagccacaat ccttggctga agctgagcaa cttctcaatc
     7201 aacacgctgc catccgtgaa gagattgacg gatacgctga ggattacaag aagatgcgtg
     7261 caatgggaga tcgtgtcact caagatcaaa ccgatccaca atacatgttc cttcgtcaac
     7321 gtcttgctgg acttcaagaa ggatgggaag agttgcagcg aatgtgggac aacagacaac
     7381 atttgctttc tcaaggactc aaccttcaaa tgttccttcg cgatgccaaa caagcagagg
     7441 ttatgctctc tcaacaagaa aactatctcg ccaaggacga tatcccacaa tctttggagc
     7501 aagccgagaa ccaactcaag agacaccaag acttcatcac cactatggat gccaacgacg
     7561 aaaagattcg cgccgtcgga atgttcggtg atcagctctg ccaagatggt cactatgccg
     7621 ctgacaagat ccataagaag gctagaaaca ttgatgaacg tcgtggagcc aaccgcgaga
     7681 aggctcaaga agtactcaag aagctgaagg acgccttgtc tctccaacaa ttcttgtccg
     7741 actgcgacga gctgagagaa tggatcgagg agaagatgat ccgtgctcaa gatgagactt
     7801 acagagacgc caagactatc acctccaaat tcgtccgaca tcaagcattc caatccgaac
     7861 ttgctgctaa caaggaacgt cttgaccaac ttaaacatgc tgccatcaac cttggtgacg
     7921 acaagccaga gtaccacgga accattgatc cacagatcga ggaactcgct actcaatggg
     7981 atgagttgga aaagactaca gaggagaagg gacagaagtt gtttgatgct aaccgccaac
     8041 aactttacgt acaaagtatt gctgacatga aggaatgggc aacccaactt gagaacgaga
     8101 tgactcgtga agaccaacca ggagatctta ccactgtcaa tgtggccatg caaaaacaac
     8161 atttgatcga aactgaaatg atcaagaagg ctcaacacat tgatcaactc atggagatgg
     8221 agccacagct cgaggaactt cacccagacg agcttgaaaa cattaaggct catcgtttgg
     8281 cagttcaaga acaacttcaa agactccagg ctccattgga cgaccgacgt aaggcattgg
     8341 aacgcaagaa ggccgccttc caatttggtc gcgatgttga tgacgagaaa ttgtggattt
     8401 ctgagagact tgttctcgct aaagctcaaa atcttggaga aagccttcca gattgtcatc
     8461 gtctccagaa gaacctacaa cttctttcca atgaaatcga caaccacgaa ccatggatca
     8521 accagatctg taataatggt caagagttga ttgacgaagg acatgctaac ggaccagcat
     8581 tcgagaagaa gattcaagaa ctgcgcagtg cttggcaaga gttgaaggaa gccgtcaaag
     8641 acagaaaggg agatcttgga gaaagcgaga aggctcatca attcttgtat gattgcggag
     8701 aggctgaagc ctggatgagc gaacaagaat tgtacatgat gcaagacgaa agaggaaaag
     8761 acgagttctc aaccaagaac caaatcaaga aacacgaacg ccttcaatcc gatattgaca
     8821 aatttgctga tacgatccgt gctcttgcca ccaaggctca caagttcgtc gaagagaagt
     8881 caccactcac cgaacagatc caagttcgtc aagctcaaat cgagaagctc tacgcaggtc
     8941 ttcaagattt gtcgaaggag cgcagaaaga gactcgaaga gaccttggaa ctttacgctc
     9001 ttcatcgtga aatcgacgat cttcttcaat ggatcgccga caaggaagtt gttgcaggaa
     9061 gtcaagagaa cggacaagac tacgaacacg tccaaatgct tcaagagaga ttccaacagt
     9121 ttgctcgtga caccgaaaac atcggatccg aacgtgttgc caatgctaat gatggatgtg
     9181 atactctcat tggtcacgga cacactgacg ctccgactat agcattgtgg aaggattcgt
     9241 tgaatgaggc ctgggagaat cttctggagt tgatggacac tcgtgcccaa attctcgagg
     9301 cttctagact tcttcacaag ttctaccacg actgccgtga ttgcttgtca agaatcatgg
     9361 aaaagactca tgctatgcct gatgatcttg gacgtgactc aagcagtgtt ggtgcactct
     9421 ccagaaaaca tcagaactat ctcaaggata ttgctgcaat tggagaacaa gttgctcaaa
     9481 tcgaaagaga tgccgcagag cttcgagatg gttacgctgg agacaaggct cttgatatcg
     9541 gatctcgcga aagcgaggtt gtcaaggcat ggaggcatct ccgcggactc tgtgacgccc
     9601 gtacatctcg tctcatggat acttctgatc ttttcaagtt catgaatatg gtccgtgatt
     9661 tgttgttgtg gatggatgag gtgaagagag aaatgaactc acaagaacgt ccgaaggatg
     9721 tgagcggagt cgagttgctc atgaacaatc atcaatcgct caaggctgaa atcgatgctc
     9781 gcgaggagaa cttcaacgcg tgcatctcat tgggacgaga tctgctcaac agaaaacact
     9841 acgcatcaag tgaaatcgag aagaagctga tcaagttgac aaccgaaaga gccgaaatga
     9901 tgcgcagatg ggaagatcgt tgggaatatc ttcagctcat tctggaagtg taccaattcg
     9961 cccgcgacgc tgctgtcgcc gaatcttggc tcttcgccca agaaccatat cttatttcga
    10021 aggaatacgg tagaaacctc gaagaaacca tcaaactcat caagaaacac gaggcgttcg
    10081 agaaatcggc attcgcacaa gaagaacgtt tcttggcgct cgagaaattg acaacgttcg
    10141 aattgaaaga aactcaacac cgcgaggagg agaccgccaa gcgacgtggt ccagctcata
    10201 ttggaagtcc ttctaggtca actccagccg cggaaacatc tttcggcgcc caggatgatg
    10261 gagccaaaca aggagaagca ttcgaaggaa cgcttatcag aaagcacact tacgagagtc
    10321 tcgaccggaa agcggctaac cgctcatggg agaaacttta cgcagtgttg cggcagaatg
    10381 aactgtcatt ctacaaagat ccgaagcata gagatgaatc tgtgcatgga gagccaccaa
    10441 tggcactgcc aggatgctct gtcaacgtgg catcagacta tcaaaagaag aagaacgtac
    10501 tgtcgctgag gttacccatc ggcgccgagt acctattcca atgcggtagc gaagaagaca
    10561 tgcagcgatg gctcaccgag ttgcaagtcg ccacggggca agcccaactg gaagaggcta
    10621 gtagatcaca gacgctacca gcagaaggca gtgccacaaa gaagaaggga ggatttttct
    10681 ctcgaggcaa gaagtaacct gaattctgca gcatctcgcg cccgtgcctc tgacttctaa
    10741 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
    10801 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
    10861 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
    10921 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
    10981 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
    11041 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
    11101 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
    11161 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
    11221 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
    11281 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
    11341 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
    11401 tgacagaata cattcccaat ataccaaaca taactgtttg gcggccgctc gagcatgcat
    11461 ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
    11521 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    11581 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    11641 gcagcctata cgtacggcag tttaaggttt acacctataa aagagagagc cgttatcgtc
    11701 tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg gtgatccccc
    11761 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
    11821 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
    11881 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
    11941 tgatgttctg gggaatataa atgtcaggca tgagattatc aaaaaggatc ttcacctaga
    12001 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
    12061 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
    12121 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
    12181 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
    12241 tgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
    12301 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
    12361 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
    12421 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
    12481 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
    12541 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
    12601 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
    12661 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
    12721 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
    12781 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
    12841 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
    12901 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
    12961 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
    13021 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
    13081 gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc
    13141 tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg gggaaatgtg
    13201 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    13261 caataaccct gataaatgct tcaataatag cacgtgagga gggccaccat ggccaagttg
    13321 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
    13381 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
    13441 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
    13501 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
    13561 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
    13621 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
    13681 tgacacgtgc taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    13741 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    13801 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    13861 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    13921 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    13981 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    14041 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    14101 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    14161 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    14221 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    14281 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    14341 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    14401 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    14461 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    14521 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    14581 gaagcggaag
//
