LOCUS       sequence-135212-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: pcDNA-ET-I654Q
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(450..468)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(451..468)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(488..505)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    494..718
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(851..870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatct accatggact
      121 acaaggacga cgatgacaag aagcctatgt cctatgagga gaagcggcag ctcagcttgg
      181 acatcaacaa gctccccggc gagaagctgg gccgcgtggt gcacatcatc cagtcacggg
      241 agccctccct gaagaattcc aaccccgacg agattgaaca ggactttgag accctgaagc
      301 cgtccacact gcgtgagctg gagcgctatg tcacctcctg tttgcggaag aaaaggaaac
      361 ctcaagctga gaaagttgat gtgattgccg gctcctccaa gatgaagggc ttctcgtaat
      421 aactcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
      481 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
      541 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
      601 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
      661 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
      721 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
      781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      841 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      901 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      961 gaccccaaaa
//
