LOCUS       sequence-135292-        9011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression plasmid for mouse Fli1 expression; containing 
            bicistronic IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 9011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: MSCV PIG Fli1
FEATURES             Location/Qualifiers
     source          1..9011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        2791..3290
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2826..2849)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3223..3242
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3311..3910
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3311..3330)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3807..3827
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3971..4538
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3988..4540
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4132..4149)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4359..4378
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4552..4554,4555..4557,4558..5271)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4597..4618)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4858..4877)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5205..5226
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5373..5887
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6056..6072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6056..6072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6069..6091)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6080..6096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6104..6110,6111..6128,6129..6134))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6149..6170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6287..6304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6458..7046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6538..6557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7217..8008,8009..8077))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7840..7859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8078..8182)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8250..8268
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8306..8328)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8428..8447
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8641..8663
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8791..8810
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9010..9011,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctgccag catggacggg actattaagg
     1441 aggctctgtc tgtggtgagt gacgatcagt ccctttttga ttcagcatac ggagcggcag
     1501 cccatctccc caaggcagat atgactgctt cggggagtcc tgactacggg cagccccaca
     1561 aaatcaaccc cctgccaccg cagcaggagt ggatcaacca gccagtgaga gtcaatgtca
     1621 agcgggagta tgaccacatg aatggatcca gggagtctcc ggtggactgc agtgtcagca
     1681 aatgtaacaa gctggtgggc ggaggcgaag ccaaccccat gaactataat agctacatgg
     1741 atgagaagaa cggcccccct cctcccaaca tgaccaccaa cgaacggaga gtcattgtgc
     1801 ctgcagaccc cacactgtgg acacaggagc acgttcgaca gtggctggag tgggctataa
     1861 aggaatacgg attgatggag attgacactt ccttcttcca gaacatggat ggcaaggaat
     1921 tgtgtaaaat gaacaaggag gacttcctcc gagccacctc cgcctacaac acagaagtgc
     1981 tgttgtcgca cctcagttac ctcagggaaa gttcactgct ggcctataac acaacctccc
     2041 atacagacca gtcctcacga ctgaatgtca aggaagaccc ttcttatgac tctgtcagga
     2101 gaggagcatg gaacaataat atgaactctg gcctcaacaa aagtcctctc cttggaggat
     2161 cacagaccat gggcaagaac actgagcagc ggccccagcc agatccttat cagatcctgg
     2221 ggccaaccag cagccgccta gcaaaccctg ggagtgggca gatccagctg tggcagtttc
     2281 tcctggaact actgtccgac agcgccaacg ccagctgtat cacctgggag gggaccaacg
     2341 gggagttcaa aatgacggac cctgatgagg tggccaggcg ctggggagag cggaagagca
     2401 agcccaacat gaattatgac aagctgagcc gggccctccg atactactat gacaaaaaca
     2461 ttatgaccaa agtgcatggc aaaaggtatg cctacaagtt tgacttccat ggcattgccc
     2521 aggccctgca gccacatcca acagagacat ccatgtacaa gtatccctct gatatctcct
     2581 acatgccttc ctaccatgcc catcaacaga aggtgaactt tgtcccgtct cacccatcct
     2641 ccatgcctgt cacctcctcc agcttctttg gagcagcatc acaatactgg acctccccca
     2701 ctgctgggat ctatccaaac cccagtgtcc cccgccatcc taacacccac gtgccttcac
     2761 acttaggcag ctactactag gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     2821 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     2881 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     2941 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     3001 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     3061 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     3121 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     3181 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     3241 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     3301 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     3361 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     3421 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     3481 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     3541 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     3601 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     3661 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     3721 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     3781 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     3841 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     3901 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     3961 tgcatccaat tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4021 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4081 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4141 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4201 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4261 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4321 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4381 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4441 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     4501 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc
     4561 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4621 aacggccaca agttcagcgt gtctggcgag ggcgagggcg atgccaccta cggcaagctg
     4681 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4741 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     4801 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4861 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     4921 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     4981 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     5041 gcgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     5101 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     5161 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5221 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta atgaattaat
     5281 taagaattat caagcttatc gataccgtcg acctgcagcc aagcttatcg ataaaataaa
     5341 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     5401 agctagctta agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa
     5461 gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg
     5521 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc
     5581 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     5641 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     5701 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata
     5761 gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     5821 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     5881 ctttcatggg taacagtttc ttgaagttgg agaacaacat tctgagggta ggagtcgaat
     5941 attaagtaat cctgactcaa ttagccactg ttttgaatcc acatactcca atactcctga
     6001 aatagttcat tatggacagc gcagaagagc tggggagaat taattcgtaa tcatggtcat
     6061 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6121 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6241 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6301 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6361 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6421 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6481 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6541 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6601 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6661 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6721 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6781 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6841 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6901 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6961 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7021 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7081 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7141 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7201 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7261 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7321 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7381 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7441 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7501 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7561 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7621 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7681 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7741 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7801 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7861 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7921 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7981 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8041 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8101 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8161 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     8221 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     8281 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     8341 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     8401 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8461 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     8521 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     8581 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     8641 cccagtcacg acgttgtaaa acgacggcgc aaggaatggt gcatgcaagg agatggcgcc
     8701 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     8761 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     8821 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagaggc gattagtcca
     8881 atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca
     8941 gctgaagcct atagagtacg agccatagat aaaataaaag attttattta gtctccagaa
     9001 aaagggggga a
//
