LOCUS       sequence-135384-         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB107
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(41..165)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(191..208)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(191..207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(200..222)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    410..434
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taaagcctgg ggtgcctaat gcggcccctc gaggggccgc catagtgact ggatatgttg
       61 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      121 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgttgata actctagacc
      181 caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      241 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      301 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga
      361 gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat
      421 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
      481 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
      541 gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt
      601 cagtgcagcc gtcttctgaa
//
