LOCUS       sequence-135458-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains 600 bp homology arms to SS9 for one-step markerless genome 
            integration in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Bassalo MC, Garst AD, Halweg-Edwards AL, Grau WC, Domaille DW, 
            Mutalik VK, Arkin AP, Gill RT
  TITLE     Rapid and Efficient One-Step Metabolic Pathway Integration in E. 
            coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 22.
  PUBMED    27072506
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Apr 22."
COMMENT     Sequence Label: pSS9
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     563..591
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aagggcagat tgtgtcgaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
       61 tggagtattt tgttgataat ggtctgctag ttgaacggat ccatcttcaa tgttgtggcg
      121 aattttgaag ttagctttga ttccattctt ttgtttgtct gccgtgatgt atacattgtg
      181 tgagttatag ttgtactcga gtttgtgtcc gagaatgttt ccatcttctt taaaatcaat
      241 accttttaac tcgatacgat taacaagggt atcaccttca aacttgactt cagcacgcgt
      301 cttgtagttc ccgtcatctt tgaaagatat agtgcgttcc tgtacataac cttcgggcat
      361 ggcactcttg aaaaagtcat gccgtttcat atgatccgga taacgggaaa agcattgaac
      421 accataagag aaagtagtga caagtgttgg ccatggaaca ggtagttttc cagtagtgca
      481 aataaattta agggtaagtt ttccgtatgt tgcatcacct tcaccctctc cactgacaga
      541 aaatttgtgc ccattaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa
      601 aagttcttct cctttactca ttttttctac cggtacccgg ggatcctcta gagtcgacct
      661 gcaggcatgc aagcttggcg taatcatggt catggtatat ctccttcttg ggaaaccgtt
      721 gtggtctccc tggctgtaag tatcctatag gttagacttt aagtcaatac tctttttgat
      781 aaacctgttt gacatatcaa
//
