LOCUS       sequence-135509-        3608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for human DND1 with stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pENTR4-hDND1
FEATURES             Location/Qualifiers
     source          1..3608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1780..1879)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1991..2010)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2002..2811
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2069..2088)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2958..3546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3447..3466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggaa ccaattcagt cgacatgcag tccaagcggg attgtgagct
      721 gtggtgtgag agggtgaatc cagagaacaa ggcggcgctg gaggcgtggg tcagggagac
      781 aggcatccgc ctggtgcagg tgaacgggca gaggaagtat ggcgggccac ccccaggctg
      841 ggtgggcagc ccgccgccag ctgggtcaga ggtgttcatc gggcggctgc ctcaggacgt
      901 gtacgagcac cagcttatcc cgctgttcca gcgcgtgggc cgcctctacg agttccgcct
      961 gatgatgacc ttcagcggcc tgaaccgcgg cttcgcctat gcccgctaca gctcgaggcg
     1021 cggcgcgcag gccgccatcg ccacgctgca caaccatccg ctgcggccgt cctgcccgct
     1081 gctcgtgtgc cgcagcaccg agaagtgtga gctgagcgtt gacggcctgc cgccgaatct
     1141 gacccgcagc gcgctgctgc tcgcgctgca gccgctgggt cccggcttgc aggaggcgcg
     1201 gctgctgccc agccccggac cggcgcccgg gcagatcgct ctgctcaaat tcagctcgca
     1261 ccgggccgct gccatggcca aaaaggccct ggtggaaggg cagtcacacc tctgtggaga
     1321 gcaggtggct gtggagtggc tcaagccaga cctgaagcag cgacttcgcc agcagcttgt
     1381 gggtcccttc ttgcggtccc cacagccaga gggcagccag ttggctttgg caagggacaa
     1441 gttagggttc caaggggctc gggctaccct gcagttgctg tgccaacgaa tgaagctggg
     1501 cagccctgtg ttcctcacca agtgtttggg cataggacct gctggctggc accgcttctg
     1561 gtaccaggtg gtgattcctg ggcatccggt gcccttcagc ggcctcatct gggttgtgct
     1621 gaccctagat ggccgggatg ggcatgaggt ggccaaggat gctgtgtctg tacggctgct
     1681 gcaggcactc agtgagtctg gggccaacct cctgtggtct gctggggctg aggcaggtac
     1741 catggttaaa cagtgagcgg ccgcactcga gatatctaga cccagctttc ttgtacaaag
     1801 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1861 aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga
     1921 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1981 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     2041 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2101 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2161 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2221 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2281 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2341 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2401 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2461 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2521 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2581 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2641 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2701 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2761 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2821 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2881 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2941 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3001 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3061 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3121 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3181 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3241 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3301 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3361 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3421 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3481 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3541 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3601 cacatgtt
//
