LOCUS       sequence-135521-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Sema3B protein (truncated at cleavage site P1; ie, 
            short), C-terminally fused to alkaline phosphatase + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3b(S)-AP-His
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taagatctct agattaatgg tgatggtgat gatgacccgg gtgcgcggcg tcggtggtgc
       61 cggcgggggg cgccaggtcg caggcggtgt agggctccag gcaggcggcg aaggccatga
      121 cgtgcgctat gaaggtctgc tcctgcacgc cgtgaaccag gtgcgcctgc gggccgcgcg
      181 cgaacaccgc cacgtcctcg cctgcgtggg tctcttcgtc caggggcact gctgactgct
      241 gccgatactc ggggctcccg ctctcgctct cggtaacatc cggccgggcg ccgtccttga
      301 gcacatagcc tggaccgttt ccgtatagga ggaccgtgta ggccttcctg tcccgggcct
      361 tgccaggggc cagcccgaag atggagctcc ctcgcagggg gtagcctccg aaggagaaga
      421 cgtgggagtg gtcggcagtg acgaggctca gcgtgtcctc ctcgctggtg agctggcccg
      481 ccctctcaat ggcgtcgtcg aacatgatcg tctcagtcag tgcccggtaa gccctgcttt
      541 catgatgacc atggtcgatg cgaccaccct ccacgaagag gaagaagccg cgggggttcc
      601 tgctcagcag gcgcagggca gcctctgtca tctccatcag ggaggggtcc agtgtggagt
      661 ctcggtggat ctcgtatttc atgtctccag gctcaaagag acccatgaga tgggtcacag
      721 acgggtccag ggaagcctgc atgagctcag tgcggttcca cacataccgg gcacc
//
