LOCUS       sequence-135524-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human dysferlin full length tagged with His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Xu L, Pallikkuth S, Hou Z, Mignery GA, Robia SL, Han R
  TITLE     Dysferlin forms a dimer mediated by the C2 domains and the 
            transmembrane domain in vitro and in living cells.
  JOURNAL   PLoS One. 2011;6(11):e27884. doi: 10.1371/journal.pone.0027884. Epub
            2011 Nov 14.
  PUBMED    22110769
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(11):e27884. doi"; pages: " 
            10.1371/journal.pone.0027884. Epub 2011 Nov 14"
COMMENT     Sequence Label: pHis6-hDF
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atgagccatc accatcacca tcacgcgtcg
       61 ctggttccgc gtggatcccc aggaattctg cagtcgacgg taccgatgct gagggtcttc
      121 atcctctatg ccgagaacgt ccacacaccc gacaccgaca tcagcgatgc ctactgctcc
      181 gcggtgtttg caggggtgaa gaagagaacc aaagtcatca agaacagcgt gaaccctgta
      241 tggaatgagg gatttgaatg ggacctcaag ggcatccccc tggaccaggg ctctgagctt
      301 catgtggtgg tcaaagacca tgagacgatg gggaggaaca ggttcctggg ggaagccaag
      361 gtcccactcc gagaggtcct cgccacccct agtctgtccg ccagcttcaa tgcccccctg
      421 ctggacacca agaagcagcc cacaggggcc tcgctggtcc tgcaggtgtc ctacacaccg
      481 ctgcctggag ctgtgcctct gttcccgccc cctactcctc tggagccctc cccgactctg
      541 cctgacctgg atgtagtggc agacacagga ggagaggaag acacagagga ccagggactc
      601 actggagatg aggcggagcc attcctggat caaagcggag gcccgggggc tcccaccacc
      661 ccaaggaaac taccttcacg tcctccgccc cactaccccg ggatcaaaag aaagcgaagt
      721 gcgcctacat ctagaaagct gctgtcagac aaaccgcagg atttccagat cagggtccag
      781 gtgatcgag
//
