LOCUS       sequence-135566-        9497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Contactin 3 protein 
            (truncated immediately upstream of propeptide cleavage and 
            GPI-attachement site), C-terminally fused to alkaline phosphatase + 
            6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Cntn3-AP-His
FEATURES             Location/Qualifiers
     source          1..9497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6077..6094
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6120..6241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6157..6176)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6211..6230
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6279..6296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6450..7038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6530..6549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7209..8000,8001..8069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7832..7851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8070..8174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8242..8260
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8298..8320)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8322..8342)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8327..8657
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8508..8643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8570..8589
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8794..8813
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8880..9335)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9017..9038)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9229..9248
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9461..9483
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9475..9492
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9476..9492
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgatgttgtc
     1621 atggaaacag ttgatcctgc tttcattcat tggctgcctg gcaggtgaac ttcttttaca
     1681 aggccctgtg tttatcaaag agcccagcaa cagtattttc cctgttgatt cagaggacaa
     1741 aaaaataaca ttaaattgtg aagcaagagg caacccttca cctcattaca ggtggcagct
     1801 gaatggcagt gatattgata caagtctgga ccatcgttac aaactgaatg gaggaaatct
     1861 catagttatt aaccctaaca gaaattggga tacaggaagt taccagtgtt ttgcaacgaa
     1921 ttctcttgga acaatagtga gcagagaagc caaactccag tttgcctatc ttgaaaattt
     1981 taagaccagg atgagaagca ctgtgtcggt ccgtgaaggc cagggagtgg tgcttctctg
     2041 tggaccccca cctcactcag gagaactgtc atatgcttgg gtcttcaatg agtatccatc
     2101 atttgttgaa gaagacagtc gacgatttgt ctctcaagag acaggacacc tctacatagc
     2161 taaggtggaa ccatctgatg tgggaaatta cacatgtgtg gtgaccagca cggtgacaaa
     2221 taccagagta ttgggctccc caaccccttt ggtgctacgc tctgatggtg tcatgggtga
     2281 atatgaaccc aaaatagaag tccagtttcc agagactctt ccagcagcta aaggttcaac
     2341 tgtgaggttg gaatgcttcg ccctgggaaa ccctgttcct cagattaatt ggagaagaag
     2401 tgatgggatg ccgtttccca acaaaattaa gttgaggaag ttcaatggta tgctggaaat
     2461 tcaaaacttc caacaggaag atacaggctc ctatgagtgt attgctgaaa attcacgagg
     2521 aaaaaatgtg gcgagagggc ggctcactta ctatgcaaaa ccttattggc ttcaactctt
     2581 aagagatgtg gaaattgctg tggaggacag tctttattgg gaatgccggg caagtgggaa
     2641 gcctaaaccc tcttatcgat ggctgaaaaa tggagatgcc ttagtactag aggagagaat
     2701 acagatagaa aatggtgccc ttacaataac caacctcaat gtgactgact ctggaatgtt
     2761 ccaatgtata gcagaaaaca agcatggtct catctactcc agtgctgagc tcaaggtagt
     2821 agcttctgct ccagattttt caagaaaccc tatgaaaaaa atggtccagg tgcaggtggg
     2881 cagcctggtg atcctggatt gtaaacccag agcctctcca agggcacttt ctttctggaa
     2941 gaagggagac atgatggtgc gggagcaggc aagggtttct tttttaaatg atggaggact
     3001 caaaatcatg aatgtgacta aggcagacgc tggaacctac acctgcacag cagaaaacca
     3061 gtttgggaaa gcaaatggca caacccactt ggttgtgaca gaacctacga gaataatttt
     3121 ggcaccttca aacatggacg ttgctgttgg tgagagcgtc attttaccct gccaggttca
     3181 acatgatccc ctattagaca taatgtttgc ctggtatttc aatggagccc ttacagactt
     3241 taagaaagat ggatctcact ttgagaaagt tggtgggagt tcatctggtg atttaatgat
     3301 cagaaacatt caattgaaac acagtgggaa gtacgtatgt atggtgcaga caggggtgga
     3361 cagtgtttcc tctgcagctg agctcatcgt gagaggttca cctgggccac cggaaaatgt
     3421 gaaggtagat gaaattacag acacaacagc ccagctctct tggacggaag gcacggacag
     3481 tcatagccca gttatatcgt atgcagtcca agctaggacg cctttctctg tgggctggca
     3541 aagtgtcaga acagtacctg aggtcattga tggcaagaca catacagcta ctgtcgtgga
     3601 gttaaaccca tgggtggaat atgaatttcg cattgtagcc agtaacaaaa tcggaggtgg
     3661 agaacccagt ttaccctcag aaaaagtaag gactgaagag gcagctccag aaatcgcacc
     3721 ttctgaggtc agcggaggag gtggaagccg atctgaacta gtgataacct gggatccagt
     3781 ccctgaagaa ctacagaatg gaggaggttt tgggtatgtg gttgctttcc gcccacttgg
     3841 ggttaccacc tggatccaga cagtggtgac atccccagat aacccaagat acgtctttag
     3901 gaatgaaagc attgtgccct tttcgccata tgaagttaaa gtgggagttt acaataacaa
     3961 aggtgaagga ccctttagcc cagtgacaac tgtgttctct gcagaggaag agcctacagt
     4021 tgccccatct cacatctctg cccatagcct gtcttcctca gaaattgaag tttcttggaa
     4081 tacaattccc tggaagttga gcaatggaca tttactgggc tatgaggtgc ggtactggaa
     4141 caatggagga gaagaagaat catcccgcaa agtgaaagtg gcaggaaatc agacatcagc
     4201 tgtgctccga ggcctgaaga gcaacctggc ctattacaca gctgtccggg cttacaacag
     4261 cgcaggcgct ggtcccttca gcgccacagt taatgcgacc accaagaaaa cccctcccag
     4321 tcagccacca ggaaatgttg tttggaatgc tacagacact aaagtgctac ttaattggga
     4381 acaagtgaaa gccatggaga atgaatcaga agtaacaggc tacaaggttt tctataggac
     4441 tagcagtcag aataatgtgc acgtgctgaa cacaaataaa acttcagctg aactgttgct
     4501 gcctatcaaa gaagactaca ttattgaggt caaggccacc acagatggag gggatggcac
     4561 cagtagtgag cagatcagga ttccaagaat aactagtgga tcttccggaa tcatcccagt
     4621 tgaggaggag aacccggact tctggaaccg cgaggcagcc gaggccctgg gtgccgccaa
     4681 gaagctgcag cctgcacaga cagccgccaa gaacctcatc atcttcctgg gcgatgggat
     4741 gggggtgtct acggtgacag ctgccaggat cctaaaaggg cagaagaagg acaaactggg
     4801 gcctgagata cccctggcca tggaccgctt cccatatgtg gctctgtcca agacatacaa
     4861 tgtagacaaa catgtgccag acagtggagc cacagccacg gcctacctgt gcggggtcaa
     4921 gggcaacttc cagaccattg gcttgagtgc agccgcccgc tttaaccagt gcaacacgac
     4981 acgcggcaac gaggtcatct ccgtgatgaa tcgggccaag aaagcaggga agtcagtggg
     5041 agtggtaacc accacacgag tgcagcacgc ctcgccagcc ggcacctacg cccacacggt
     5101 gaaccgcaac tggtactcgg acgccgacgt gcctgcctcg gcccgccagg aggggtgcca
     5161 ggacatcgct acgcagctca tctccaacat ggacattgac gtgatcctag gtgggggccg
     5221 aaagtacatg tttcgcatgg gaaccccaga ccctgagtac ccagatgact acagccaagg
     5281 tgggaccagg ctggacggga agaatctggt gcaggaatgg ctggcgaagc gccagggtgc
     5341 ccggtatgtg tggaaccgca ctgagctcat gcaggcttcc ctggacccgt ctgtgaccca
     5401 tctcatgggt ctctttgagc ctggagacat gaaatacgag atccaccgag actccacact
     5461 ggacccctcc ctgatggaga tgacagaggc tgccctgcgc ctgctgagca ggaacccccg
     5521 cggcttcttc ctcttcgtgg agggtggtcg catcgaccat ggtcatcatg aaagcagggc
     5581 ttaccgggca ctgactgaga cgatcatgtt cgacgacgcc attgagaggg cgggccagct
     5641 caccagcgag gaggacacgc tgagcctcgt cactgccgac cactcccacg tcttctcctt
     5701 cggaggctac cccctgcgag ggagctccat cttcgggctg gcccctggca aggcccggga
     5761 caggaaggcc tacacggtcc tcctatacgg aaacggtcca ggctatgtgc tcaaggacgg
     5821 cgcccggccg gatgttaccg agagcgagag cgggagcccc gagtatcggc agcagtcagc
     5881 agtgcccctg gacgaagaga cccacgcagg cgaggacgtg gcggtgttcg cgcgcggccc
     5941 gcaggcgcac ctggttcacg gcgtgcagga gcagaccttc atagcgcacg tcatggcctt
     6001 cgccgcctgc ctggagccct acaccgcctg cgacctggcg ccccccgccg gcaccaccga
     6061 cgccgcgcac ccgggtcatc atcaccatca ccattaatct agagatctta ttaaagcaga
     6121 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6181 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6241 atcatgtctg gtcgactcta gctagactct tccgcttcct cgctcactga ctcgctgcgc
     6301 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6361 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6421 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6481 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6541 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6601 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6661 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6721 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6781 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6841 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6901 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6961 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7021 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7081 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7141 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7501 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7801 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8041 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8101 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8161 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     8221 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     8281 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg aggaatgtgt gtcagttagg
     8341 gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt
     8401 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8461 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8521 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8581 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8641 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgtc ccggagacgg
     8701 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8761 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8821 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     8881 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     8941 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     9001 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     9061 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     9121 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     9181 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     9241 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     9301 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
     9361 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     9421 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     9481 acgacggcca gtgaatt
//
