LOCUS       sequence-135582-       13839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module composed of 3 transcriptional units: Luciferase and Renilla 
            proteins, and the Tomato bushy stunt virus (TBSV) silencing 
            suppressor P19 plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13839)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 13839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB3
            Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos...
FEATURES             Location/Qualifiers
     source          1..13839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..262
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             297..1949
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(363..381)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      1976..2228
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3400..3744
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3817..4752
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     terminator      4779..5031
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5875..6219
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      6834..7086
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7489..7513
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8814..9443
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9877..10944
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      11010..11204
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11440..11462)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11548..11688
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11603..11622
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11703..11720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11874..12462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11954..11973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12550..13365)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(12550..13365)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     13273..13292
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    13798..13822
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagatacat gagaattaag ggagtcacgg tatgaccccc gccgatgacg cgggacaagc
       61 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
      121 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
      181 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
      241 aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc gaggcaatgg
      301 aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg
      361 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
      421 ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc
      481 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
      541 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
      601 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc
      661 agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
      721 agctcccaat catccaaaaa attattatca tggattctaa aacggattac cagggatttc
      781 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
      841 tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta
      901 ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg
      961 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     1021 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     1081 tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga
     1141 ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg
     1201 acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg
     1261 aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc
     1321 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     1381 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     1441 tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg
     1501 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     1561 acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga
     1621 ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc
     1681 ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg
     1741 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     1801 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     1861 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     1921 ccaagaaggg cggaaagatc gccgtgtaat gagcttggaa tggatcttcg atcccgatcg
     1981 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgacgat
     2041 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     2101 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     2161 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     2221 actagatcgg gaattgccaa gctaattctt gaagacgaaa gggcctcgtg atacgcctat
     2281 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2341 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2401 tcatgagaca ataaccctga taaatgcttc aataatggga ccgactcgcg ctgtcaggag
     2461 agcgaaatga ttgatgaaga acaatggtgg atgaagaaca aagaaggagg gagcttttgt
     2521 tcaagatgaa caaagaacaa tagtggatga agaacaaagt gaaaaaaata aaaaaaaatg
     2581 tatggttaaa taaagagtaa agttaccatt gagactcctg acagcgaaat gattgatgaa
     2641 gaacaatggt ggatgaagaa caaagaagga gggagctttt gttcaagatg aacaaagaac
     2701 aatagtggat gaagaacaaa gtgaaaaaaa taaaaaaaaa tgtatggtta aataaagagt
     2761 aaagttacca ttgagactcc gtcaggagac tagagccaag ctgatctcct ttgccccgga
     2821 gatcaccatg gacgactttc tctatctcta cgatctagga agaaagttcg acggagaagg
     2881 tgacgatacc atgttcacca ccgataatga gaagattagc ctcttcaatt tcagaaagaa
     2941 tgctgaccca cagatggtta gagaggccta cgcggcaggt ctgatcaaga cgatctaccc
     3001 gagtaataat ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag
     3061 attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac
     3121 tattccagta tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg
     3181 agtctctaag aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga
     3241 ggatctaaca gaactcgccg tgaagactgg cgaacagttc atacagagtc ttttacgact
     3301 caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctcg tctactccaa
     3361 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     3421 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     3481 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     3541 tcaagatgcc cctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     3601 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
     3661 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     3721 aagttcattt catttggaga ggactccggt atttttacaa caataccaca acaaaacaaa
     3781 caacaaacaa cattacaatt tactattcta gtcgaaatga cttcgaaagt ttatgatcca
     3841 gaacaaagga aacggatgat aactggtccg cagtggtggg ccagatgtaa acaaatgaat
     3901 gttcttgatt catttattaa ttattatgat tcagaaaaac atgcagaaaa tgctgttatt
     3961 tttttacatg gtaacgcggc ctcttcttat ttatggcgac atgttgtgcc acatattgag
     4021 ccagtagcgc ggtgtattat accagacctt attggtatgg gcaaatcagg caaatctggt
     4081 aatggttctt ataggttact tgatcattac aaatatctta ctgcatggtt tgaacttctt
     4141 aatttaccaa agaagatcat ttttgtcggc catgattggg gtgcttgttt ggcatttcat
     4201 tatagctatg agcatcaaga taagatcaaa gcaatagttc acgctgaaag tgtagtagat
     4261 gtgattgaat catgggatga atggcctgat attgaagaag atattgcgtt gatcaaatct
     4321 gaagaaggag aaaaaatggt tttggagaat aacttcttcg tggaaaccat gttgccatca
     4381 aaaatcatga gaaagttaga accagaagaa tttgcagcat atcttgaacc attcaaagag
     4441 aaaggtgaag ttcgtcgtcc aacattatca tggcctcgtg aaatcccgtt agtaaaaggt
     4501 ggtaaacctg acgttgtaca aattgttagg aattataatg cttatctacg tgcaagtgat
     4561 gatttaccaa aaatgtttat tgaatcggac ccaggattct tttccaatgc tattgttgaa
     4621 ggtgccaaga agtttcctaa tactgaattt gtcaaagtaa aaggtcttca tttttcgcaa
     4681 gaagatgcac ctgatgaaat gggaaaatat atcaaatcgt tcgttgagcg agttctcaaa
     4741 aatgaacaat aatgagcttg gaatggatct tcgatcccga tcgttcaaac atttggcaat
     4801 aaagtttctt aagattgaat cctgttgccg gtcttgcgac gattatcata taatttctgt
     4861 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     4921 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     4981 gcgcaaacta ggataaatta tcgcgcdcgg tgtcatctat gttactagat cgggaattgc
     5041 caagctaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5101 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5161 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5221 tgataaatgc ttcaataatg ggaccgactc gcgctgtcag gagactagag ccaagctgat
     5281 ctcctttgcc ccggagatca ccatggacga ctttctctat ctctacgatc taggaagaaa
     5341 gttcgacgga gaaggtgacg ataccatgtt caccaccgat aatgagaaga ttagcctctt
     5401 caatttcaga aagaatgctg acccacagat ggttagagag gcctacgcgg caggtctgat
     5461 caagacgatc tacccgagta ataatctcca ggagatcaaa taccttccca agaaggttaa
     5521 agatgcagtc aaaagattca ggactaactg catcaagaac acagagaaag atatatttct
     5581 caagatcaga agtactattc cagtatggac gattcaaggc ttgcttcata aaccaaggca
     5641 agtaatagag attggagtct ctaagaaagt agttcctact gaatcaaagg ccatggagtc
     5701 aaaaattcag atcgaggatc taacagaact cgccgtgaag actggcgaac agttcataca
     5761 gagtctttta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
     5821 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
     5881 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
     5941 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
     6001 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     6061 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     6121 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     6181 ttcctctata taaggaagtt catttcattt ggagaggact ccggtatttt tacaacaata
     6241 ccacaacaaa acaaacaaca aacaacatta caatttacta ttctagtcga aatggaacga
     6301 gctatacaag gaaacgacgc tagggaacaa gctaacagtg aacgttggga tggaggatca
     6361 ggaggtacca cttctccctt caaacttcct gacgaaagtc cgagttggac tgagtggcgg
     6421 ctacataacg atgagactaa ttcgaatcaa gataatcccc ttggtttcaa ggaaagctgg
     6481 ggtttcggga aagttgtatt taagagatat ctcagatacg acaggacgga agcttcactg
     6541 cacagagtcc ttggatcttg gacgggagat tcggttaact atgcagcatc tcgatttttc
     6601 ggtttcgacc agatcggatg tacctatagt attcggtttc gaggagttag tatcaccgtt
     6661 tctggaggct ctcgaactct tcagcatctc tgtgagatgg caattcggtc taagcaagaa
     6721 ctgctacagc ttgccccaat cgaagtggaa agtaatgtat caagaggatg ccctgaaggt
     6781 actgaaacct tcgaaaaaga aagcgagtga gcttggaatg gatcttcgat cccgatcgtt
     6841 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta
     6901 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     6961 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     7021 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     7081 tagatcggga attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt
     7141 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7201 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7261 atgagacaat aaccctgata aatgcttcaa taatgggacc gactcgcgct gtcaggagtc
     7321 tcaatggtaa ctttactctt tatttaacca tacatttttt tttatttttt tcactttgtt
     7381 cttcatccac tattgttctt tgttcatctt gaacaaaagc tccctccttc tttgttcttc
     7441 atccaccatt gttcttcatc aatcatttcg ctgtcatgag acgaattctg acaggatata
     7501 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     7561 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     7621 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     7681 agtgcagccg tcatctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     7741 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     7801 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     7861 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     7921 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     7981 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     8041 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     8101 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     8161 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     8221 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     8281 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     8341 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     8401 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     8461 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     8521 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     8581 gcggccgggt acgtgttcga gccgcccgcg cacctctcaa ccgtgcggct gcatgaaatc
     8641 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     8701 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     8761 gtcgctgcgt atatgatccg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     8821 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgga acccatctag
     8881 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     8941 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     9001 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     9061 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     9121 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     9181 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     9241 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     9301 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     9361 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     9421 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     9481 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     9541 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     9601 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatagat
     9661 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     9721 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     9781 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     9841 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta
     9901 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     9961 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
    10021 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
    10081 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
    10141 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
    10201 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
    10261 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
    10321 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
    10381 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
    10441 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
    10501 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
    10561 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
    10621 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
    10681 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
    10741 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
    10801 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
    10861 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
    10921 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
    10981 ggggaaaaag gtcgaaaagg actctttcct gtggatagca cgtacattgg gaacccaaag
    11041 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
    11101 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
    11161 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
    11221 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
    11281 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
    11341 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
    11401 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    11461 ggtgacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    11521 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    11581 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    11641 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
    11701 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    11761 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    11821 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    11881 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    11941 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    12001 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    12061 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    12121 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    12181 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    12241 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    12301 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    12361 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    12421 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    12481 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
    12541 tctaggtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
    12601 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
    12661 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
    12721 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
    12781 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
    12841 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
    12901 tcgtgattgc gcctgagcga gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac
    12961 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
    13021 atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa
    13081 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
    13141 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
    13201 tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg tcgcacctga
    13261 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
    13321 taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacag aacttattat
    13381 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    13441 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    13501 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
    13561 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
    13621 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
    13681 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
    13741 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
    13801 caggatatat tgtggtgtaa acataacgaa ttcgtctca
//
