LOCUS       sequence-135584-       12041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for human codon optimized with mutated (D10A, 
            H840A) and inactivated catalytic domains Cas9 protein plant 
            expression driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12041)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 12041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB 35S:dCas9:Tnos (GB1191)
FEATURES             Location/Qualifiers
     source          1..12041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     904..924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1034..1060,1061..1063,1064..3547,3548..3553,3554..3619,
                     3620..3622,3623..5137)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1034..1060,1061..1063,1064..3550,3551..3553,
                     3554..5137)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1034..5137
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5150..5170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5197..5449
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5484..5502)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5686..5710
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7011..7640
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8074..9141
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      9207..9401
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9637..9659)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9745..9885
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9800..9819
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9900..9917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10071..10659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10151..10170)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10747..11562)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10747..11562)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11470..11489
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    11995..12019
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     1021 tattctagtc gaaatggaca agaagtactc cattgggctc gctatcggca caaacagcgt
     1081 cggctgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg
     1141 caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg
     1201 ggaaacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa
     1261 gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc
     1321 tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca
     1381 cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata
     1441 tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct
     1501 cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc
     1561 agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt
     1621 cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct
     1681 gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg
     1741 cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt
     1801 cgacctggcc gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga
     1861 caatctgctg gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct
     1921 gtcagacgcc attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc
     1981 gctgagcgct agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa
     2041 ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa
     2101 aaatggctac gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat
     2161 taagcccatc ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga
     2221 agatctgttg cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct
     2281 gggcgaactg cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa
     2341 cagggaaaag attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc
     2401 ccggggaaat tccagattcg cgtggatgac tcgcaaatca gaagagacta tcactccctg
     2461 gaacttcgag gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac
     2521 taactttgat aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga
     2581 gtacttcaca gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa
     2641 gccagcattc ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa
     2701 ccggaaagtt accgtgaaac agctcaaaga agattatttc aaaaagattg aatgtttcga
     2761 ctctgttgaa atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga
     2821 tctcctgaaa atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct
     2881 tgaggacatt gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt
     2941 gaaaacttac gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata
     3001 tacaggatgg gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg
     3061 aaagacaatc ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt
     3121 gatccatgat gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca
     3181 gggggacagt ctccacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg
     3241 aatactgcag accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc
     3301 cgagaatatc gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa
     3361 cagtagggaa aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct
     3421 taaggaacac ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct
     3481 gcagaacggc agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta
     3541 cgacgtggat gccatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt
     3601 gttgacaaga tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt
     3661 caagaaaatg aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa
     3721 gttcgataat ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt
     3781 catcaaaagg cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga
     3841 ttcacgcatg aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat
     3901 tactctgaag tctaagctgg tttcagattt cagaaaggac tttcagtttt ataaggtgag
     3961 agagatcaac aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc
     4021 acttatcaaa aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta
     4081 cgatgttagg aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta
     4141 cttcttttac agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga
     4201 gattcggaag cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa
     4261 gggtagggat ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa
     4321 aaagaccgaa gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag
     4381 cgacaagctg atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc
     4441 tcctacagtc gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa
     4501 actcaaaagc gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa
     4561 aaaccccatc gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat
     4621 taagcttccc aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag
     4681 tgcgggcgag ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt
     4741 gtatctggcc agccactatg aaaagctcaa aggatctccc gaagataatg agcagaagca
     4801 gctgttcgtg gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt
     4861 ctccaaaaga gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa
     4921 gcacagggat aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac
     4981 caacttgggc gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta
     5041 cacctctaca aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta
     5101 tgaaacaaga atcgacctct ctcagctcgg tggagacagc agggctgacc ccaagaagaa
     5161 gaggaaggtg tgagcttgga atggatcttc gatcccgatc gttcaaacat ttggcaataa
     5221 agtttcttaa gattgaatcc tgttgccggt cttgcgacga ttatcatata atttctgttg
     5281 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     5341 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     5401 gcaaactagg ataaattatc gcgcdcggtg tcatctatgt tactagatcg ggaattgcca
     5461 agctaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5521 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5581 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5641 ataaatgctt caataatggg accgactcgc gcttgagacg aagcttgaca ggatatattg
     5701 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     5761 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     5821 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     5881 gcagccgtca tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     5941 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     6001 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     6061 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     6121 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     6181 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     6241 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     6301 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     6361 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     6421 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
     6481 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     6541 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     6601 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     6661 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     6721 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     6781 gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg
     6841 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     6901 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     6961 gctgcgtata tgatccgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     7021 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcggaacc catctagccc
     7081 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     7141 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     7201 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     7261 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     7321 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     7381 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     7441 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     7501 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     7561 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     7621 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     7681 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     7741 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     7801 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatagatcgc
     7861 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     7921 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     7981 gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca
     8041 ctggaacccc caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa
     8101 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     8161 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     8221 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     8281 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     8341 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     8401 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     8461 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     8521 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     8581 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     8641 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     8701 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     8761 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     8821 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     8881 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     8941 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     9001 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     9061 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     9121 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     9181 gaaaaaggtc gaaaaggact ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     9241 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     9301 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     9361 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     9421 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     9481 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     9541 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     9601 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccggt
     9661 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     9721 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     9781 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     9841 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     9901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    10021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10201 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10261 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10321 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10381 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10441 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10501 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
    10741 aggtgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
    10801 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
    10861 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
    10921 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
    10981 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
    11041 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
    11101 tgattgcgcc tgagcgagtc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
    11161 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
    11221 aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg tgagtaacca
    11281 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
    11341 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
    11401 cagaaacaac tctggcgcat cgggcttccc atacaatcgg tagattgtcg cacctgattg
    11461 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
    11521 tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacagaac ttattatttc
    11581 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    11641 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    11701 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
    11761 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
    11821 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
    11881 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
    11941 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
    12001 gatatattgt ggtgtaaaca taacaagctt cgtctcagtc a
//
