LOCUS       sequence-135615-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP160 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC154-dual-dCas9VP160-sgExpression
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccgcctct ctcggccttg gtcagattgt cgaactttct ctgggtaatc agcttggcgt
       61 tcagcagctg ccgccagtag ttcttcatct tcttcacgac ctcttcggag ggcacgttgt
      121 cgctcttgcc ccggttcttg tcgcttctgg tcagcacctt gttgtcgatg gagtcgtcct
      181 tcagaaagct ctgaggcacg atggcgtcca catcgtagtc ggacagccgg ttgatgtcca
      241 gttcctggtc cacgtacata tcccgcccat tctgcaggta gtacaggtac agcttctcgt
      301 tctgcagctg ggtgttttcc acggggtgtt ctttcaggat ctggctgccc agctctttga
      361 tgccctcttc gatccgcttc attctctcgc ggctgttctt ctgtcccttc tgggtggtct
      421 ggttctctct ggccatttcg atcacgatgt tctcgggctt gtgccggccc atcactttca
      481 cgagctcgtc caccaccttc actgtctgca ggatgccctt cttaatggcg gggctgccgg
      541 ccagattggc aatgtgctcg tgcaggctat cgccctggcc ggacacctgg gctttctgga
      601 tgtcctcttt aaaggtcagg ctgtcgtcgt ggatcagctg catgaagttt ctgttggcga
      661 agccgtcgga cttcaggaaa tccaggattg tcttgccgga ctgcttgtcc cggatgccgt
      721 tgatcagctt ccggctcagc ctgccccagc cggtgtatct ccgccgcttc agctgcttca
      781 tcactttgtc
//
