LOCUS       sequence-135701-       15342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FlpO for  circuit mapping and genetic manipulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15342)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 15342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-FlpO
FEATURES             Location/Qualifiers
     source          1..15342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6771..6782
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8574..8585)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11782..11800)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11796..11843
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11871..11887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11871..11887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11884..11906)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11895..11911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11919..11925,11926..11943,11944..11949))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11964..11985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12102..12119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12273..12861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12353..12372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13032..13823,13824..13892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13655..13674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13893..13997)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14024..14479
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14111..14130)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14321..14342
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14605..14627
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14619..14636
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14620..14636
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        14706..15009
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15010..15213
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15164..15184
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     15258..15277
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15258..15276
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatgagc cagttcgaca tcctgtgcaa gacccccccc
     3361 aaggtgctgg tgcggcagtt cgtggagaga ttcgagaggc ccagcggcga gaagatcgcc
     3421 agctgtgccg ccgagctgac ctacctgtgc tggatgatca cccacaacgg caccgccatc
     3481 aagagggcca ccttcatgag ctacaacacc atcatcagca acagcctgag cttcgacatc
     3541 gtgaacaaga gcctgcagtt caagtacaag acccagaagg ccaccatcct ggaggccagc
     3601 ctgaagaagc tgatccccgc ctgggagttc accatcatcc cttacaacgg ccagaagcac
     3661 cagagcgaca tcaccgacat cgtgtccagc ctgcagctgc agttcgagag cagcgaggag
     3721 gccgacaagg gcaacagcca cagcaagaag atgctgaagg ccctgctgtc cgagggcgag
     3781 agcatctggg agatcaccga gaagatcctg aacagcttcg agtacaccag caggttcacc
     3841 aagaccaaga ccctgtacca gttcctgttc ctggccacat tcatcaactg cggcaggttc
     3901 agcgacatca agaacgtgga ccccaagagc ttcaagctgg tgcagaacaa gtacctgggc
     3961 gtgatcattc agtgcctggt gaccgagacc aagacaagcg tgtccaggca catctacttt
     4021 ttcagcgcca gaggcaggat cgaccccctg gtgtacctgg acgagttcct gaggaacagc
     4081 gagcccgtgc tgaagagagt gaacaggacc ggcaacagca gcagcaacaa gcaggagtac
     4141 cagctgctga aggacaacct ggtgcgcagc tacaacaagg ccctgaagaa gaacgccccc
     4201 taccccatct tcgctatcaa gaacggccct aagagccaca tcggcaggca cctgatgacc
     4261 agctttctga gcatgaaggg cctgaccgag ctgacaaacg tggtgggcaa ctggagcgac
     4321 aagagggcct ccgccgtggc caggaccacc tacacccacc agatcaccgc catccccgac
     4381 cactacttcg ccctggtgtc caggtactac gcctacgacc ccatcagcaa ggagatgatc
     4441 gccctgaagg acgagaccaa ccccatcgag gagtggcagc acatcgagca gctgaagggc
     4501 agcgccgagg gcagcatcag ataccccgcc tggaacggca tcatcagcca ggaggtgctg
     4561 gactacctga gcagctacat caacaggcgg atctgagcta gcggcgccta gccggtcatc
     4621 cttttgacga ttccagtccc gaggataacc tcctctcggg attgggggga atctttggat
     4681 ccagtagtcc tccttgaact ccgtccaaca gggcagattc aagagtcata agactttcat
     4741 taatcatttc agttgatcag acatggtcgt gtagattctc ttaatacggg agatcttcta
     4801 gcagtttcag tgaccaacgg tgctttcatt ctccaggaac tgataccaaa ggttgtggac
     4861 aggccaaggg gtacttcgga tgactctgtg cttgggcaca gaaagaggtc gtagtgtgcc
     4921 ccccgatagc ggactcgaca tgaatcaact aagaaaggca atctgcctcc catgatggac
     4981 ataagcaata gttcacaacc atcttgcatc tcagtgaagt gtacataact attgagggct
     5041 gggtcatcta agcatttcag tcgagaaaaa aactgtagac caaaagaaca actagcaaca
     5101 cttctcatcc agagacccat atcaagatgc tagatccggg agaggtttat gatgacccta
     5161 ttgatccaat tgagtcagag gctgaaccca gaggaacccc cactgtcccc aacatcttga
     5221 ggaactccga ctacaatctc aattctcctt tgatagagga tcctgccaaa ctaatgttag
     5281 aatggttgaa gacagggaac agaccttatc ggatgacttt gacagacaat tgctccaggt
     5341 cttacaaagt tttgaaagat tatttcaaga aagtagattt gggttctctc aaagtgggcg
     5401 gaactgctgc acagtcaatg gtttctctct ggttgtgtgg tgcccactct gaatcaaaca
     5461 ggagccggag atgtataacc gacttggccc atttctattc caagtcatcc cccatagaga
     5521 agctattgaa ttgtacgcta ggaaacagag gcctgagaat cccaccagag ggggtgttaa
     5581 attgcctcga gagggtcaat tatgacaagg catttgggag gtatctggcc aacacgtatt
     5641 cctcttactt gtttttccat gtaatcacct tatacatgaa tgccttagac tgggaagagg
     5701 aaaagaccat cctggcatta tggaaagata taacctcagt ggataccgag aaggacttgg
     5761 tcaaattcaa agatcaaata tggggactgt tgattgtgac aaaggacttt gtttactctc
     5821 agagttcaaa ctgtcttttt gacagaaact acacactgat gctaaaggat cttttcttgt
     5881 ctcgattcaa ctccttaatg attctgcttt ctccccctga gccccgatac tcagatgact
     5941 taatatctca gctgtgccag ctatacatcg ctggggatca agtcttgtcc atgtgtggga
     6001 actccggcta tgaagtcatc aaaatattgg agccatatgt cgtgaacagt ttggtccaga
     6061 gggcagagaa gtttaggcct ctcatccacc ccttgggaga ctttcctatg ttcataaaag
     6121 acaaggtgaa tcaacttgaa gggacttttg gtcccagtgc aaagaggttt tttagggttt
     6181 tagatcaatt cgacaacata catgacttag tatttgtgta tggctgttac agacattggg
     6241 ggcaccccta tatagattat cggaagggtc tgtcgaaact atatgatcaa gttcacatta
     6301 agaaagtaat agataagtcc taccaggagt gtttagcaag tgacttggcc agaaggatcc
     6361 tcagatgggg atttgacaag tactccaaat ggtatctaga ttcgagattc cttgcccgag
     6421 accacccctt gactccttat gtcaagaccc aaacatggcc acccaaacat atagtagact
     6481 tggtggggga cacatggcat aagctcccga tcacgcagat ctttgaaatt cctgaatcaa
     6541 tggacccgtc agagatactg gatgacaaat cacattcttt caccagaaca aggttagctt
     6601 cttggctgtc agagaaccga ggggggcctg ttcctagcga gaaggtcatt atcacggccc
     6661 tgtctaagcc acctgtcaat ccccgagagt ttttgaaatc tatagacctc ggaggattgc
     6721 cagatgagga tttgataatt ggcctcaaac caaaggaacg ggagttgaag atcgagggcc
     6781 gattctttgc tctaatgtca tggaatctaa gattatattt tgtcatcacc gaaaagctct
     6841 tggccaacta cattttgcca ctttttgacg cactgactat gacagacaac ctgaacaagg
     6901 tgttcaaaaa gttgatcgac agggtcaccg ggcaagggct tttggactat tctagggtca
     6961 catacgcatt tcacctggac tatgagaaat ggaacaatca tcaaagattg gagtcaacag
     7021 aggatgtatt ctctgtccta gatcaggtgt ttggattgaa gagggtgttt tctagaacac
     7081 acgagttttt tcagaagtcc tggatctatt attcagacag atcagacctc attgggttat
     7141 gggaggacca aatatattgc ttggatatgt ctaacggccc aacctgctgg aatggccaag
     7201 atggcgggct agagggcttg cggcagaagg gctggagtct agtcagttta ttaatgatag
     7261 atagagaatc tcaaaccagg aacacaagaa ccaagatact agctcaagga gacaaccagg
     7321 ttctgtgtcc tacatacatg ttgtcaccgg gattgtctca agaggggctt ctctatgagt
     7381 tagagagcat atcaaggaat gcactctcaa tataccgagc tatcgaggaa ggagcatcta
     7441 agctggggct gatcatcaag aaggaagaga ccatgtgtag ttatgacttt ctcatatatg
     7501 ggaagacccc cttatttcga ggcaacatat tggtgcctga atccaaaaga tgggcccgag
     7561 tctcttgcat ctctaacgac caaatagtca acctcgccaa tataatgtcg acagtatcca
     7621 ccaatgcgct gacagtggca caacactctc aatctctgat caaacctatg agggattttc
     7681 tgctcatgtc agtacaggca gttttccact acctgttgtt tagcccaatc ctaaaaggca
     7741 gagtttataa gattctgagt gctgaagggg agagctttct cctagccatg tcgcggataa
     7801 tctacctaga tccttctttg ggaggggtgt ctggaatgtc tctcgggagg ttccatatac
     7861 gtcagttctc agaccctgtc tctgaagggt tgtcattctg gagagagatc tggttaggct
     7921 ctcatgagtc ctggattcac gcgttgtgtc aggaggccgg gaaccccgat cttggagaga
     7981 gaacactcga gagcttcact cgccttttag aagatcctac taccttaaat atcaaaggag
     8041 gggccagccc taccattcta ctcaaggatg ctatcagaaa ggctctgtac gacgaggtgg
     8101 acaaggtgga aaattcagag tttcgagagg caatcctgct gtccaagacc catagagata
     8161 actttatact ctttttaaaa tctgttgagc ctctgttccc tcgatttctc agtgagctct
     8221 tcagttcgtc tttcttggga ataccggagt caatcattgg actgatacaa aactcccgga
     8281 caataagaag gcagtttaga aagagtctct caagaacttt agaagagtcc ttctacaact
     8341 cagagatcca cgggattaat cggataaccc agacacctca aagggtcgga agggtgtggc
     8401 cttgctcttc agagagggcg gatctactta gggagatctc ttgggggagg aaagtggtag
     8461 gcacgacagt tcctcaccct tccgagatgt tggagttgtt tcccaaatcc tccatttcct
     8521 gcacttgtgg agcaacaggg ggaggcaatc ctagagtctc tgtatcagta ctcccgtcct
     8581 tcgatcagtc atttttctca cggggccccc taaagggata cttgggctcg tccacctcta
     8641 tgtcaaccca gctattccat gcatgggaaa aagtcactaa tgttcatgtg gtgaaaaggg
     8701 ctctatcgtt aaaagaatct ataaactggt tcatcaatag gaattccaat ttggctcaaa
     8761 ctctaattgg aaacatcatg tctctgacag gccctgactt ccctctagaa gaggcccctg
     8821 ttttcaaacg gacagggtca gccttgcata ggttcaagtc tgccagatac agcgaaggag
     8881 ggtattcttc tgtttgccct aaccttctct cccatatctc tgttagtaca gacactatgt
     8941 ctgatttgac ccaaaacggg aagaactatg atttcatgtt tcagccattg atgctttatg
     9001 cgcaaacatg gacatcggaa ctggtacaga gggatacaag acttagagac tccacgtttc
     9061 actggcacct tcggtgcaac agatgtgtga ggcccattga tgatataaca ctggaaactt
     9121 ctcagatctt cgagttcccg gatgtgtcaa aaaggatatc caggatggtt tctggagctg
     9181 tccctcaatt tcagaagctt cctgatatcc gtctaagacc aggtgatttt gaatccctaa
     9241 gtggtagaga aaagtctcgc catatagggt cagctcaggg gctcttatac tcaatcttag
     9301 tagcaattca cgactcagga tacaatgatg ggaccatctt ccctgtcaac atatacggca
     9361 aagtttcccc cagagactat ttgagagggc ttgcaagagg gatcttgata gggtcctcga
     9421 tttgcttctt gacacgaatg acaaatatta acattaacag acctcttgaa ttgatctcag
     9481 gggtaatttc ctatattctc ctgaggctgg ataatcatcc ctctctgtat ataatgctca
     9541 gagaaccgtc tcttagagga gaaatattct ctatccctca gaaaatcccc gccgcttacc
     9601 caaccactat gagagaaggc aacagatcga ttttgtgtta cctccaacac gtgctacgct
     9661 atgagcgaga ggcaatcacg gcgtccccgg agaatgactg gctgtggatc ttctcagact
     9721 tcagaagtgt gaaaatgacg tacttgaccc tcattactta ccagtctcac ctcctactcc
     9781 agagggttga gagaaacttg tctaagagta tgagagctac tctgcgacaa atgggttcct
     9841 taatgaggca agtgctgggt gggcacggag aagatacctt ggagtcagac gatgacattc
     9901 aacgattact aaaagactct ttgcgaaaga caaggtgggt ggatcaagag gtgcgccatg
     9961 cagctagaac catgaatgga gattacagcc ccgacaagaa agtatcccgt aaggtaggat
    10021 gttcagaatg ggtctgctct gctcaacaga ttgccgtctc cacctcagcc aacccggccc
    10081 ctgtctcaga gcttgacatt agggccctct ctaagcggtt tcaaaacccc ttgatctcgg
    10141 gcctgagagt ggttcagtgg gcaaccggtg cccattataa gcttaagcct attctagatg
    10201 atctaaatgt tttcccatct ctctgtcttg taattggaga cgggtcaggg ggaatatcaa
    10261 gggcagttct caacatgttt ccagattcta agcttgtgtt caacagccta ttggaggtga
    10321 atgatctgat ggcttccgga acacatccac tgcctccttc agcaatcatg agtggaggag
    10381 atgacatcat ctccagagtg atagactttg actcaatatg ggagaaaccg tccgacctga
    10441 ggaactcggc cacctggaga tacttccagt cggttcaaaa acaggtcaac atgtcgtatg
    10501 acctcattat ttgtgatgca gaagttactg atattgcatc tatcaaccgg ataactctgt
    10561 tgatgtctga tttcgcattg tctatagatg gaccacttta tctggtcttc aaaacttacg
    10621 ggactatgct agtaaacccg gactataaag ctattcaaca tctgtcaaga gcgttccctt
    10681 cggtcacagg gtttgtaacc caagtaactt catccttttc ttctgagcta tacctccggt
    10741 tctctaaacg aggaaagttt ttcagggacg ccgagtactt gacctcttcc acccttcgag
    10801 agatgagcct tgtgttgttc aattgcagca gccccaaaag tgagatgcag agagctcgtt
    10861 ccttaaacta tcaagacctg gtaaggggat ttcctgaaga gatcatatca aatccttaca
    10921 acgaaatgat cataactctg attgacaatg atgtagagtc cttcctagtc cacaagatgg
    10981 tggatgatct tgagctacag aggggaactc tgtctaaagt ggctatcatt atatccatca
    11041 tgatcgtttt ttccaataga gtcttcaaca tttccaaacc tttgactgac cccttgttct
    11101 atccaccgtc tgatcctaaa atcctgaggc acttcaacat atgttgcagt actatgatgt
    11161 atctatctac cgctttaggc gacgtcccta acttcgcaag acttcatgac ctgtataaca
    11221 gacctataac ttgttacttc agaaaacaag ttattcgagg gaatatttat ctatcttgga
    11281 gttggtccga tgatacccca gtgttcaaga gagtagcctg taattctagc ttgagtctgt
    11341 catctcactg gatcaggttg atctacaaga tagtgaagac taccagactc attggcagca
    11401 taaaagacct atcaggagag gtagaacgac atcttcatgg gtataacaga tggatcaccc
    11461 tcgaggatat ccgatctaga tcatccctac tagattacag ttgtttgtaa gccggatatt
    11521 accgaaagcc tgtgcatgct aaaattcttg tatgatgcat cttgaaaaaa acaagatctt
    11581 gaatccggac ctctggttgt ttgattgttt tttccatctt tattgttttt ttgttaagcg
    11641 tgggtcggca tggcatctcc acctcctcgc ggtccgacct gggcatccga aggaggacgc
    11701 acgtccactc ggatggctaa gggaggggcg gggatccggc tgctaacaaa gcccgaaagg
    11761 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
    11821 aacgggtctt gaggggtttt ttgctgaaag tcgcgcttgg cgtaatcatg gtcatagctg
    11881 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    11941 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    12001 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    12061 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    12121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    12181 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    12241 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    12301 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    12361 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    12421 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    12481 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12541 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12601 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    12661 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    12721 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12781 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12841 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    12901 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12961 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    13021 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    13081 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    13141 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    13201 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    13261 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    13321 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    13381 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    13441 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    13501 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    13561 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    13621 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    13681 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    13741 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13801 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13861 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    13921 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13981 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
    14041 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    14101 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    14161 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    14221 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    14281 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    14341 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    14401 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    14461 atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    14521 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    14581 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
    14641 gcgcctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
    14701 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    14761 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    14821 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    14881 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    14941 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    15001 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
    15061 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
    15121 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
    15181 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
    15241 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagattaag
    15301 cgtctgatga gtccgtgagg acgaaacccg gcgtaccggg tc
//
