LOCUS       sequence-135868-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pT181 STAR Antisense Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL2154
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     377..395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(399..442)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        460..494
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(613..630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(864..883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
      361 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
      421 agctttcgct aaggatgatt tctggaattc taaagatctt tgacagctag ctcagtccta
      481 ggtataatac tagtaacaaa ataaagcaat aaggaatcgc tcacccaaat atatatcttt
      541 atgtggatct caaagcccgc cgaaaggcgg gctttttttt ggatccttac tcgagtctag
      601 actgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      661 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      721 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      781 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      901 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      961 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1021 ctcca
//
