LOCUS       sequence-135882-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pT181 Sense Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL2148
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..235
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(454..500)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     653..670
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(726..743)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(759..788)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
       61 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
      121 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
      181 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
      241 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
      301 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
      361 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactgc agtctagact
      421 cgagtaagga tccagttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
      481 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac
      541 actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac
      601 cgcgctactg ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat
      661 ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag ccaagctgga gaccgtttaa
      721 actcaatgat gatgatgatg atggtcgacg gcgctattca gatcctcttc tgagatgagt
      781 ttttgttcgg gcccaagctt cagatcctta tttgtagagc tcatccatgc catgtgtaat
      841 cccagcagca gttacaaa
//
