LOCUS       sequence-135883-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pT181 Sense Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL2148
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        186..220
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(439..467)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
       61 acctgaagtc agccccatac gatataagtt gtaattctca tgtttgacag cttatcatcg
      121 ataagcttcc gatggcgcgc cgagaggctt tacactttat gcttccggct gaattctaaa
      181 gatctttgac agctagctca gtcctaggta taatactagt acataaagat atatatttgg
      241 gtgagcgatt ccttaaacga aattgagatt aaggagtcgc tctttttttt ttatgtataa
      301 aaacaatcat gcaaatcatt caaatcattt ggaaaatcac gatttagaca atttttctaa
      361 aaccggctac tctaatagcc ggttgtaagg atctaggagg aaggatctat gagcaaagga
      421 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      481 cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa actcaccctt
      541 aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt cactactctg
      601 acctatggtg ttcaatgctt ttcccgttat ccggatcaca tgaaacggca tgactttttc
      661 aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg
      721 acctacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag
      781 ttaaagggta ttgattttaa agaagatgga aacattcttg gacacaaact cgagtacaac
      841 tttaactcac acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac
      901 ttcaaaattc gccacaacgt tgaagatggt tccgttcaac tagcagacca ttatcaacaa
      961 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa
     1021 tctgtccttt cgaaagatcc caacgaaaa
//
