LOCUS       sequence-135902-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses suppression tRNA and mCherry inserted with a Npu DnaE 
            intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Ren W, Ji A, Ai HW
  TITLE     Light activation of protein splicing with a photocaged fast intein.
  JOURNAL   J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. 
            Epub 2015 Feb 5.
  PUBMED    25647354
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja508597d";
            journalName: "J Am Chem Soc"; date: "2015-02-18- 18"; volume: "137";
            issue: "6"; pages: "2155-8"
COMMENT     Sequence Label: pMAH2-intein/mCherry
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        221..239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     221..238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     252..271
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      304..313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(455..473)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga
      121 gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttcg ttagaacgcg
      181 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
      241 taacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcacggaag
      301 cttgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      361 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      421 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      481 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      541 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaat
      601 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      661 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      721 ggccccgtaa tgcagtagtt aagctatgaa acggaaatat tgacagtaga atatggatta
      781 ttaccgattg gtaaaattgt
//
