LOCUS       sequence-135970-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit with N-terminal His6 tag (H6AaPurS); 
            hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK522
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             341..358
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             784..801
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(854..872)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      868..915
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgaa ggtacgtgta accgtcatgc
      121 tgaaggacgg tgttctggac ccgcagggca aggccatcag ccacgcattg cagacacttg
      181 gttttgaagg cgtgcacgat gtgcgcgtgg gtaaggtgat tgaactggat ctgccgggtg
      241 ccaaagccga ggaagcccag aaaaaggccg atgccatggc gcgtgatctg ctagccaacc
      301 tggtgattga agattactct gtagaggtgg tggaatgagc caccaccacc accaccacaa
      361 gcgcaccctt cttatagcgc tggcattggc atgcgcggca ccgggcttgg cccatgccca
      421 gcgcattgcg cccatgaagg caggtaactt tgcccgcctt tgtacgcgcg ccactagtgc
      481 tggcatttgc gatgcctata ttggcggcct gacggatggg gtctctcttt ccaaaatcaa
      541 tgatcggaac gagggggatc cttctgcgcc tgctggtttt tgtgttcccg ctacagaaac
      601 catgcccgtt atgcgctcca aggtacttac ctggctgaaa gcgcattctg aaacactgga
      661 caagccggtt ggtgaaagtg tttttgccgc cctgcacgcc tcttatccgt gcagtgtgaa
      721 aaaatgaaag cagcaattat cgtattcccc ggtaccctcg agaagcttgc ggccgcactc
      781 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
      841 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      901 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
      961 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     1021 ccagcgcc
//
