LOCUS       sequence-136064-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains the CORE cassette with convergent KlURA3 and KanMX4 
            markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Storici F, Resnick MA
  TITLE     Delitto perfetto targeted mutagenesis in yeast with 
            oligonucleotides.
  JOURNAL   Genet Eng (N Y). 2003;25:189-207.
  PUBMED    15260239
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genet Eng 
            (N Y)."; date: "2003"; volume: "25"; pages: "189-207"
COMMENT     Sequence Label: pCORE-UK
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      75..272
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
       61 tttttctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
      121 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
      181 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
      241 tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc
      301 cagtgtcgaa aacgagctcc accgcggtgg cggccgcttg ttgttcctta ccattaagtt
      361 gatccattgt gtgcttgctt cttttcttat ccggggtata tgataattaa tcttaccaat
      421 tggatgacaa acagtctgga ggaagtttga gagggcttat cgcaatggtt gtaatggttt
      481 aaaactcttt ttcgatgatg tagtttctgg tttttaaatc tcacataatg aaagagagag
      541 agagaagcaa acaaaaaatt ttcagttcaa tacaacagat cacgtgatct ttttgtaaga
      601 tgaagttgaa gtagtgttgc accgtgccaa tgcaggtggc tattagatta aatatgtgat
      661 ttgttctatt aagtttcctg tataattaat ggggagcgct gattctcttt tggtacgctt
      721 cccatccagc atttctgtat ctttcacctt caaccttagg atctctaccc ttggcgaaaa
      781 gtcctctgcc aacaatgatg atatctgatc caccacttac aacttcgtcg acggttctgt
      841 actgctgacc caatgcatcg cctttgtcgt ctaaacctac acctggggtc atgattagcc
      901 aatcaaaccc ttcttctctt cctcccatat cgttctgagc aatgaaccca ataacgaaat
      961 ctttatcact ctttgcaata tcaacggtac ccttagtata ttcaccgtgt gctagagaac
     1021 ccttggaaga caattcagca agcatcaata atccccttgg ttctttggtg acctcttg
//
