LOCUS       sequence-136176-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Franciscella tularensis N5-carboxyaminoimidazole 
            ribonucleotide mutase with N-terminal TEV protease-cleavable His6 
            tag (H6tFtPurE1), synthetic gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK569
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(578..598)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(623..640)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             651..673
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    688..712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(712..731)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(713..731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(781..798)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(781..797)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(790..812)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1006..1025)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc aagcttgtcg
       61 acggagctcg aattcggatc cgttagtgct ccctaggatc aggattttcc aaaacaaatc
      121 gtgtttgctc agctctaaac tcagctagtg cttttgcaat atttatatct gtatgttgca
      181 agatacttgc agcaaaaagc gccgcgtttt ttgctcctgc cataccaatt gcaaaagtag
      241 ctactggaat acccgctggc atctgtacta ttgataataa actatcttgg ccattaagtg
      301 tacttgattt aactggtaca cctaatactg gtaaagtagt ttttgccgca accattccag
      361 gtaaatgtgc agcaccaccc gcgccagcga tgatgacttt cagaccgcgt tctttcgccg
      421 tttctgcgta atcgaacatt ttgtccggcg tacgatgcgc gctaaccact tcgcattcat
      481 aaccgatgcc caggttgtcc agaatatcac agcattcttt catggtactc caatcagatt
      541 tgctgcccat aatgacgcca acctgaactg acatgttaga ctgaaagtac agattttcgg
      601 tgcccagatc cacgccgctt gaatgatgat gatgatggtg catatgtata tctccttctt
      661 aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt
      721 gagtcgtatt aatttcattg ggtcatctag atgcattcgc gaggtaccga gctcgaattc
      781 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      841 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      901 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      961 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     1021 tgccgcatag ttaagccagc cccgacaccc gcca
//
