LOCUS       sequence-136179-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Escherichia coli evgA (Plac noncoding).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Kappock
  TITLE     Additional E. coli YfdE/EvgA constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK641
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(972..988)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(972..988)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(985..1007)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    996..1012
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gaattcgtga ttttccttaa gcttctaaga ctaaaccgtg gcttttgcaa tacaattctt
       61 acgcctgtag gattagtaag aagacttata gtgccaactt gaaactataa atcatcggta
      121 caatccctga ttttattgtt gacatttcat ttatgccgac tatttatatg gtatacttgt
      181 cgaattatct taaaggaagc tcagattttc ttatttttat tgagaaaatg agatgacgcc
      241 ttatgtctgt attactacag ggagaaggga gatgcttcat tgcaaaggga ataatctatg
      301 aacgcaataa ttattgatga ccatcctctt gctatcgcag caattcgtaa tttattgatc
      361 aaaaacgata ttgaaatctt agcagagttg actgaaggcg gaagtgccgt tcagcgggtg
      421 gaaacactta agcctgatat cgtcatcatt gatgtcgata tccccggagt taacggtatc
      481 caggtgttag aaacgctgag gaagcgccaa tatagcggaa ttattattat cgtctccgct
      541 aaaaatgacc atttttacgg gaaacattgt gctgatgctg gcgctaatgg tttcgtgagt
      601 aaaaaagaag gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa tggctactgc
      661 tatttcccct tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca gcaaaaactc
      721 gactccttat cgaaacaaga aattagtgtc atgcggtata ttcttgatgg caaggataat
      781 aatgacattg ctgaaaaaat gttcatcagc aacaaaactg tcagcactta taaaagtcgc
      841 ctgatggaaa aattagaatg taaatcactg atggatcttt acacattcgc acaacgtaac
      901 aaaatcggct aagaattcaa ggaaaatctg aattcgtcga cctgcaggca tgcaagcttg
      961 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     1021 aacatacgag ccggaa
//
