LOCUS       sequence-136181-        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Escherichia coli evgA (Plac coding).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Kappock
  TITLE     Additional E. coli YfdE/EvgA constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK646
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(889..905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(889..905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(902..924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    913..929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(937..943,944..961,962..967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    982..1003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttgtgcgaat gtgtaaagat ccatcagtga tttacattct aatttttcca tcaggcgact
       61 tttataagtg ctgacagttt tgttgctgat gaacattttt tcagcaatgt cattattatc
      121 cttgccatca agaatatacc gcatgacact aatttcttgt ttcgataagg agtcgagttt
      181 ttgctggtcg gacgttaaac ttccaacaaa ccggttgaga gagaagggga aatagcagta
      241 gccatttttt gcagcttcaa tagccgcaat gatattgttc atgccttctt ttttactcac
      301 gaaaccatta gcgccagcat cagcacaatg tttcccgtaa aaatggtcat ttttagcgga
      361 gacgataata ataattccgc tatattggcg cttcctcagc gtttctaaca cctggatacc
      421 gttaactccg gggatatcga catcaatgat gacgatatca ggcttaagtg tttccacccg
      481 ctgaacggca cttccgcctt cagtcaactc tgctaagatt tcaatatcgt ttttgatcaa
      541 taaattacga attgctgcga tagcaagagg atggtcatca ataattattg cgttcataga
      601 ttattccctt tgcaatgaag catctccctt ctccctgtag taatacagac ataaggcgtc
      661 atctcatttt ctcaataaaa ataagaaaat ctgagcttcc tttaagataa ttcgacaagt
      721 ataccatata aatagtcggc ataaatgaaa tgtcaacaat aaaatcaggg attgtaccga
      781 tgatttatag tttcaagttg gcactataag tcttcttact aatcctacag gcgtaagaat
      841 tgtattgcaa aagccacggt ttagtcttag aagcttggcg taatcatggt catagctgtt
      901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      961 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1021 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaac
//
