LOCUS       sequence-136183-         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola ATCC 35405 PurE with an N-terminal His6
            tag (H6TdPurE2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK616
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgcg tccgctggtg attatcctta
      121 tgggcagcag ttcagatatg ggtcacgccg agaaaattgc gtctgaattg aaaacttttg
      181 gtattgagta tgccattcgc atcggctccg cgcataaaac agcggagcat gtagtgtcta
      241 tgctgaaaga atatgaagct ttggaccgtc ctaaactgta catcacgatc gctgggcgta
      301 gtaacgcttt atcaggcttt gttgatggct tcgtcaaagg tgcaaccatt gcctgcccac
      361 cgccaagcga tagcttcgcc ggcgccgaca tttattcttc gttacggatg ccgagcggga
      421 ttagtccggc actggttctt gaaccgaaaa atgcggcact cctggcggct cgtatttttt
      481 cactgtacga taaagaaatc gcagattcgg tcaaatccta tatggaatcc aatgcgcaga
      541 aaattatcga agacgattcg aaactgaaac gctgaattca gcgtttcagt ttcttgtcga
      601 cgga
//
