LOCUS       sequence-136204-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPIN4
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             799..816
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gactcgtagg aacatcgtgg gctgccgcat
      121 tcaacacggc tggaaggaag gcaacgagcc agtggagcag tggaagggta ctgtgctcga
      181 gcaggtttcc gtgaagccca ctctttacat cattaaatat gatggcaaag atagtgtgta
      241 tggactagaa ctgcaccgcg ataagagagt tttagcgcta gagatccttc ctgagagagt
      301 gccaactcct cgtatcgatt cacgactggc agattccctg attggcaagg cagtggagca
      361 tgtgtttgaa ggtgaacatg gtaccaagga tgaatggaag ggtatggtcc tggcgcgagc
      421 tcctgtgatg gatacttggt tttacatcac ctacgagaaa gatcctgttc tctatatgta
      481 cacgctgctt gatgactaca aagatggtga cttacgcatt attccagatt ccaactacta
      541 tttccctaca gcagaacagg agcctggaga ggtggtcgac agtctcgtgg gcaagcaggt
      601 ggagcatgcc aaagatgacg ggtccaagag aactggcatt tttatacatc aagtggtggc
      661 gaagccatct gtttacttca ttaagtttga tgatgatatt cacatttatg tctatggttt
      721 ggtgaaaact ccttgacagt aaaggtggat acggatccga attcgagctc cgtcgacaag
      781 cttgcggccg cactcgagca ccaccaccac caccactga
//
