LOCUS       sequence-136214-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SMARCA2
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..37
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..82
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             514..531
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(584..602)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      598..645
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(769..788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agaaggagat atacatatgc accatcatca tcatcattct tctggtgtag atctgggtac
       61 cgagaacctg tacttccaat ccatggcgga aaaactgagc ccgaacccgc cgaaactgac
      121 caaacagatg aacgcgatta ttgataccgt tattaactat aaagatagca gcggtcgcca
      181 gctgagcgaa gtgtttattc agctgccgag ccgtaaagaa ctgccggaat attatgaact
      241 gattcgcaaa ccggtggatt ttaaaaaaat taaagaacgt attcgtaacc ataaatatcg
      301 tagcctgggt gatctggaaa aagatgtgat gctgctgtgc cataatgccc agacctttaa
      361 cctggaaggt agccagattt atgaagatag catcgtgctg cagagcgtgt ttaaaagcgc
      421 gcgccagaaa attgccaaag aagaagaatg acagtaaagg tggatacgga tccgaattcg
      481 agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      541 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      601 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      661 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      721 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      781 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      841 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      901 gtgatggttc acgtagtggg ccatcgccct gatagacgg
//
