LOCUS       sequence-136221-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RHOA
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             706..723
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(776..794)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      790..837
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(961..980)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat ggctgccatc cggaagaaac tggtgattgt
      121 tggtgatgga gcctgtggaa agacatgctt gctcatagtc ttcagcaagg accagttccc
      181 agaggtgtat gtgcccacag tgtttgagaa ctatgtggca gatatcgagg tggatggaaa
      241 gcaggtagaa ttggctttgt gggacacagc tgggcaggaa gattatgatc gcctgaggcc
      301 cctctcctac ccagataccg atgttatact gatgtgtttt tccatcgaca gccctgatag
      361 tttagaaaac atcccagaaa agtggacccc agaagtcaag catttctgtc ccaacgtgcc
      421 catcatcctg gttgggaata agaaggatct tcggaatgat gagcacacaa ggcgggagct
      481 agccaagatg aagcaggagc cggtgaaacc tgaagaaggc agagatatgg caaacaggat
      541 tggcgctttt gggtacatgg agtgttcagc aaagaccaaa gatggagtga gagaggtttt
      601 tgaaatggct acgagagctg ctctgcaagc tagacgtggg tgacagtaaa ggtggatacg
      661 gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac
      721 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      781 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      841 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      901 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      961 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtca
//
