LOCUS       sequence-136249-        5269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human Syndecan-1 serine 15 and 25 mutated to alanine 
            (numbering excludes signal peptide) in HEK293-EBNA1 (293E) 
            suspension culture. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Zhang Y, Wang N, Raab RW, McKown RL, Irwin JA, Kwon I, van Kuppevelt
            TH, Laurie GW
  TITLE     Targeting of heparanase-modified syndecan-1 by prosecretory mitogen 
            lacritin requires conserved core GAGAL plus heparan and chondroitin 
            sulfate as a novel hybrid binding site that enhances selectivity.
  JOURNAL   J Biol Chem. 2013 Apr 26;288(17):12090-101. doi: 
            10.1074/jbc.M112.422717. Epub 2013 Mar 15.
  PUBMED    23504321
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.422717"; journalName: "J Biol Chem"; date: 
            "2013-04-26- 26"; volume: "288"; issue: "17"; pages: "12090-101"
COMMENT     Sequence Label: pTT5-hSDC1-S15/25A
FEATURES             Location/Qualifiers
     source          1..5269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     576..596
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     622..646
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1127..1146
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    2181..2236
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2235..2254)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3319..3337)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3405..3509
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3510..3578,3579..4370)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3728..3747)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4541..5129
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5030..5049
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagaggg
     1201 cccgtttctg ctagcaagct tatgaggcgc gcggcgctct ggctctggct gtgcgcgctg
     1261 gcgctgagcc tgcagctggc cctgccgcaa attgtggcta ctaatttgcc ccctgaagat
     1321 caagatggcg ctggggatga ctctgacaac ttctccggcg caggtgcagg tgctttgcaa
     1381 gatatcacct tgtcacagca gaccccctcc acttggaagg acacgcagct cctgacggct
     1441 attcccacgt ctccagaacc caccggcctg gaggctacag ctgcctccac ctccaccctg
     1501 ccggctggag aggggcccaa ggagggagag gctgtagtcc tgccagaagt ggagcctggc
     1561 ctcaccgccc aggagcagga ggccaccccc cgacccaggg agaccacaca gctcccgacc
     1621 actcatcagg cctcaacgac cacagccacc acggcccagg agcccgccac ctcccacccc
     1681 cacagggaca tgcagcctgg ccaccatgag acctcaaccc ctgcaggacc cagccaagct
     1741 gaccttcaca ctccccacac agaggatgga ggtccttctg ccaccgagag ggctgctgag
     1801 gatggagcct ccagtcagct cccagcagca gagggctctg gggagcagga cttcaccttt
     1861 gaaacctcgg gggagaatac ggctgtagtg gccgtggagc ctgaccgccg gaaccagtcc
     1921 ccagtggatc agggggccac gggggcctca cagggcctcc tggacaggaa agaggtgctg
     1981 ggaggggtca ttgccggagg cctcgtgggg ctcatctttg ctgtgtgcct ggtgggtttc
     2041 atgctgtacc gcatgaagaa gaaggacgaa ggcagctact ccttggagga gccgaaacaa
     2101 gccaacggcg gggcctacca gaagcccacc aaacaggagg aattctatgc ctgaggatcc
     2161 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     2221 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     2281 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     2341 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     2401 gccaactgaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga
     2461 ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt
     2521 tagtaaaagg gtcctaagga acagcgatgt aggtgggcgg gccaagatag gggcgcgatt
     2581 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     2641 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     2701 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     2761 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     2821 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     2881 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     2941 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     3001 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     3061 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     3121 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     3181 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     3241 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaatta
     3301 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3361 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3421 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3481 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3541 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3601 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3661 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3721 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     3781 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3841 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3901 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3961 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4021 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     4081 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4141 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4201 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4261 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4321 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4381 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4441 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4501 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4561 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4621 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4681 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4741 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4801 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4861 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4921 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4981 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5041 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5101 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5161 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5221 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccg
//
